Document Detail


Cellular and clinical impact of haploinsufficiency for genes involved in ATR signaling.
MedLine Citation:
PMID:  17564965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ataxia telangiectasia and Rad3-related (ATR) protein, a kinase that regulates a DNA damage-response pathway, is mutated in ATR-Seckel syndrome (ATR-SS), a disorder characterized by severe microcephaly and growth delay. Impaired ATR signaling is also observed in cell lines from additional disorders characterized by microcephaly and growth delay, including non-ATR-SS, Nijmegen breakage syndrome, and MCPH1 (microcephaly, primary autosomal recessive, 1)-dependent primary microcephaly. Here, we examined ATR-pathway function in cell lines from three haploinsufficient contiguous gene-deletion disorders--a subset of blepharophimosis-ptosis-epicanthus inversus syndrome, Miller-Dieker lissencephaly syndrome, and Williams-Beuren syndrome--in which the deleted region encompasses ATR, RPA1, and RFC2, respectively. These three genes function in ATR signaling. Cell lines from these disorders displayed an impaired ATR-dependent DNA damage response. Thus, we describe ATR signaling as a pathway unusually sensitive to haploinsufficiency and identify three further human disorders displaying a defective ATR-dependent DNA damage response. The striking correlation of ATR-pathway dysfunction with the presence of microcephaly and growth delay strongly suggests a causal relationship.
Authors:
Mark O'Driscoll; William B Dobyns; Johanna M van Hagen; Penny A Jeggo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-17
Journal Detail:
Title:  American journal of human genetics     Volume:  81     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-13     Completed Date:  2007-09-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  77-86     Citation Subset:  IM    
Affiliation:
Genome Damage and Stability Centre, University of Sussex, Brighton, UK. m.o-driscoll@sussex.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Ataxia Telangiectasia / genetics
Blepharophimosis / genetics
Blepharoptosis / genetics
Cell Cycle Proteins / genetics*
Cell Line
Chromosomes, Human, Pair 7 / genetics
DNA Damage / genetics*
Dwarfism / genetics*
Gene Deletion
Haploidy*
Humans
Microcephaly / genetics*
Protein-Serine-Threonine Kinases / genetics*
RNA, Small Interfering / pharmacology
Replication Protein A / genetics
Replication Protein C / genetics
Signal Transduction / genetics
Syndrome
Williams Syndrome / genetics
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/RFC2 protein, human; 0/RNA, Small Interfering; 0/RPA1 protein, human; 0/Replication Protein A; 0/Replication Protein C; EC 2.7.1.-/ATR protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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