Document Detail


Cellular cholesterol enrichment prevents prion peptide-induced neuron cell damages.
MedLine Citation:
PMID:  20875400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prion diseases are neurodegenerative disorders characterized by the conversion of the PrPc (normal cellular prion) to the PrPsc (misfolded isoform). The accumulation of PrPsc within the central nervous system (CNS) leads to neurocytotoxicity by increasing oxidative stress. In addition, many neurodegenerative disorders including prion, Parkinson's and Alzheimer's diseases may be regulated by cholesterol homeostasis. The effects of cholesterol balance on prion protein-mediated neurotoxicity and ROS (reactive oxygen species) generation were the focus of this study. Cholesterol treatment inhibited PrP (106-126)-induced neuronal cell death and ROS generation in SH-SY5Y neuroblastoma cells. In addition, the PrP (106-126)-mediated increase of p53, p-p38, p-ERK and the decrease of Bcl-2 were blocked by cholesterol treatment. These results indicated that cellular cholesterol enrichment is a key regulator of PrP-106-126-mediated oxidative stress and neurotoxicity. Taken together, the results of this study suggest that modulation of cellular cholesterol appears to prevent the neuronal cell death caused by prion peptides.
Authors:
Jae-Kyo Jeong; Jae-Won Seol; Myung-Hee Moon; Jae-Suk Seo; You-Jin Lee; Jin-Shang Kim; Sang-Youel Park
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  401     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2010-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  516-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Chonbuk National University, Center for Healthcare Technology Development, Bio-Safety Research Institute, College of Veterinary Medicine, Jeonju, Jeonbuk 561-756, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Cell Line
Cell Line, Tumor
Cholesterol / metabolism*,  pharmacology
Humans
Neurons / drug effects,  physiology*
Peptide Fragments / metabolism*,  pharmacology
Prions / metabolism*,  pharmacology
Reactive Oxygen Species / metabolism
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/Prions; 0/Reactive Oxygen Species; 0/prion protein (106-126); 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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