Document Detail


Cellular cholesterol efflux and cholesterol loading capacity of serum: effects of LDL-apheresis.
MedLine Citation:
PMID:  22414482     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High LDL-cholesterol (LDL-C) characterizes familial hypercholesterolemia (FH) and familial combined hyperlipidemia (FCH). LDL-apheresis, used in these patients to reduce LDL-C levels, has been shown to also affect HDL levels and composition. We studied LDL-apheresis effects on six FH and nine FCH subjects' serum capacity to modulate cellular cholesterol efflux, an index of HDL functionality, and to load macrophages with cholesterol. Serum cholesterol efflux capacity (CEC) and macrophage cholesterol loading capacity (CLC) were measured before, immediately after, and two days after LDL-apheresis. The procedure reduced total cholesterol (TC), LDL-C, and apoB plasma levels (-69%, -80% and -74%, respectively), parameters only partially restored two days later. HDL-C and apoA-I plasma levels, reduced after LDL-apheresis (-27% and -16%, respectively), were restored to almost normal levels two days later. LDL-apheresis reduced serum aqueous diffusion (AD) CEC, SR-BI-CEC, and ABCA1-CEC. AD and SR-BI were fully restored whereas ABCA1-CEC remained low two days later. Sera immediately and two days after LDL-apheresis had a lower CLC than pre-LDL-apheresis sera. In conclusion, LDL-apheresis transiently reduces HDL-C levels and serum CEC, but it also reduces also serum capacity to deliver cholesterol to macrophages. Despite a potentially negative effect on HDL levels and composition, LDL-apheresis may counteract foam cells formation.
Authors:
M P Adorni; F Zimetti; M Puntoni; F Bigazzi; F Sbrana; F Minichilli; F Bernini; N Ronda; E Favari; T Sampietro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-12
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-12     Completed Date:  2012-08-08     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  984-9     Citation Subset:  IM    
Affiliation:
Department of Pharmacological and Biological Sciences and Applied Chemistries, University of Parma, Parma, Italy.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / metabolism
Antigens, CD36 / metabolism
Biological Transport
Blood Component Removal*
Cholesterol, LDL / blood*,  metabolism*
Diffusion
Female
Humans
Hyperlipoproteinemia Type II / blood,  therapy
Macrophages / metabolism
Male
Middle Aged
Time Factors
Water / metabolism
Chemical
Reg. No./Substance:
0/ABCG1 protein, human; 0/ATP binding cassette transporter 1; 0/Antigens, CD36; 0/Cholesterol, LDL; 7732-18-5/Water
Comments/Corrections

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