Document Detail


Cellular chemical indices of right ventricular protection in children.
MedLine Citation:
PMID:  6215386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myocardial preservation during open heart surgery in children was studied using biopsies of the right ventricle taken at the beginning and end of a bypass with a Tru-Cut biopsy needle. Three assessments were used: (1) semi-quantitative cytochemical grading of Baker's acid haematein reaction, and the distribution of succinate dehydrogenase and myosin ATPase; (2) microdensitometry of the succinate dehydrogenase activity; and (3) quantitative birefringence measurements to assess the response of the myocardial fibres to ATP and calcium. For each assessment, the values at the beginning and end of the bypass were compared. In a series of 42 children, two died in low cardiac output and three others required substantial inotropic support. No patient showed deterioration in the overall cytochemical assessment. Two patients showed deterioration in birefringence, one died and one had low cardiac output. The remaining three patients who had post-operative problems had low birefringence values at the beginning of bypass. Thirty-two patients were used for the microdensitometric assays, one died and three required substantial inotropic support. Succinate dehydrogenase activity decreased significantly during bypass in only two patients. One of these died and the other required substantial inotropic support.
Authors:
J Cooper; C Bull; S Canković-Darracott; J Stark
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  14     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1982-12-16     Completed Date:  1982-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  739-46     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / analysis
Biopsy
Birefringence
Child
Child, Preschool
Heart Defects, Congenital / surgery*
Heart Ventricles / enzymology
Histocytochemistry
Humans
Infant
Myocardium / enzymology*,  pathology
Succinate Dehydrogenase / analysis
Chemical
Reg. No./Substance:
EC 1.3.99.1/Succinate Dehydrogenase; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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