Document Detail


Cellular characterization of adipose tissue from various body sites of women.
MedLine Citation:
PMID:  8675558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adipose tissue is the primary site of estrogen biosynthesis in postmenopausal women. The two main histologic components of adipose tissue are mature adipocytes and fibroblasts. Aromatase P450 expressed in the fibroblast component of adipose tissue is responsible for catalyzing conversion of C19 steroids to estrogens. We previously have demonstrated that in women, aromatase expression in adipose tissue of various body sites increases with age and that aromatase expression in the hip is markedly higher than in the abdomen. To determine whether this age- and regional-dependent variation in aromatase expression is caused by an alteration in the ratio of fibroblasts to mature adipocytes, we collected sc adipose tissue samples from 19 women (age range: 21-93 yr) at the time of autopsy. Using a computerized image analysis system, we determined by morphometry the proportions of adipocytes, fibroblasts, and vascular endothelial cells within histologic sections of adipose tissue from midabdomen, both breasts, and both hips. The percentage of each cell component at each body site was expressed as the mean of triplicate replicates. Statistical analysis of our results did not indicate any correlation between advancing age and fibroblast to adipocyte ratios in the breast, abdomen, or hip. Fibroblast to adipocyte ratios were found to be significantly higher in the breast and abdomen compared with the hip ( P < 0.05). No statistical differences were found between the breast and abdomen. These findings suggest that the increase in aromatase expression with advancing age and the higher aromatase expression in the hip compared with the abdomen in women may be caused by alterations in specific signal transduction mechanisms rather than a simple increase in local adipose fibroblast numbers.
Authors:
J D Rink; E R Simpson; J J Barnard; S E Bulun
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  81     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-15     Completed Date:  1996-08-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2443-7     Citation Subset:  AIM; IM    
Affiliation:
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235-9051, USA.
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MeSH Terms
Descriptor/Qualifier:
Abdomen
Adipocytes / cytology*,  enzymology
Adipose Tissue / cytology*
Adult
Aged
Aged, 80 and over
Aromatase / analysis
Breast
Cell Count
Endothelium, Vascular / cytology
Female
Fibroblasts / cytology*,  enzymology
Hip
Humans
Middle Aged
Tissue Distribution
Grant Support
ID/Acronym/Agency:
R37-AG08174/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
EC 1.14.14.1/Aromatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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