| Cellular casein kinase II-mediated phosphorylation of rinderpest virus P protein is a prerequisite for its role in replication/transcription of the genome. | |
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MedLine Citation:
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PMID: 14993654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphoprotein P of rinderpest virus (RPV), when expressed in E. coli, is present in the unphosphorylated form. Bacterially expressed P protein was phosphorylated by a eukaryotic cellular extract, and casein kinase II (CK II) was identified as the cellular kinase involved in phosphorylation. In vitro phosphorylation of P-deletion mutants identified the N terminus as a phosphorylation domain. In vivo phosphorylation of single or multiple serine mutants of P protein identified serine residues at 49, 88 and 151 as phospho-acceptor residues. The role of P protein phosphorylation in virus replication/transcription was evaluated using the RPV minigenome system and replication/transcription of a reporter gene in vivo. P protein phosphorylation was shown to be essential for in vivo replication/transcription since phosphorylation-null mutants do not support expression of a reporter gene. Transfection of increased amounts of phosphorylation-null mutant did not support minigenome replication/transcription in vivo. |
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Authors:
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Rajnish Kaushik; M S Shaila |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general virology Volume: 85 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-02 Completed Date: 2004-04-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 687-91 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Casein Kinase II Cercopithecus aethiops DNA Replication / genetics Genes, Reporter Genome, Viral* Phosphorylation Protein-Serine-Threonine Kinases / metabolism* RNA Replicase / metabolism* Rinderpest virus / genetics*, metabolism Substrate Specificity Transcription, Genetic / genetics Vero Cells Viral Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Viral Proteins; EC 2.7.11.1/Casein Kinase II; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.7.48/RNA Replicase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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