Document Detail

Cellular binding, motion, and internalization of synthetic gene delivery polymers.
MedLine Citation:
PMID:  17888530     Owner:  NLM     Status:  MEDLINE    
Using fluorescence microscopy we have tracked the cellular binding, surface motion, and internalization of polyarginine and polyethylenimine, cationic ligands used for gene and protein delivery. Each ligand was complexed with a quantum dot to provide a photostable probe. Transfection with exogenous DNA was used to relate the observed motion to gene delivery. Cell surface motion was independent of sulfated proteoglycans, but dependent on cholesterol. Cellular internalization required sulfated proteoglycans and cholesterol. These observations suggest that sulfated proteoglycans act as cellular receptors for the cationic ligands, rather than only passive binding sites. Understanding the interaction of polyarginine and polyethylenimine with the plasma membrane may assist in designing more efficient gene delivery systems.
Gaelen T Hess; William H Humphries; Nicole C Fay; Christine K Payne
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-08-07
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1773     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-08     Completed Date:  2007-12-19     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1583-8     Citation Subset:  IM    
Biophysics Program, Harvard University, Cambridge, MA 02138, USA.
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MeSH Terms
Arginine / metabolism*
Biological Transport, Active
Cell Line
Cell Membrane / metabolism
Cholesterol / metabolism
DNA / administration & dosage
Gene Transfer Techniques*
Microscopy, Fluorescence
Polyethyleneimine / metabolism*
Polymers / metabolism*
Proteoglycans / metabolism
Quantum Dots
Grant Support
F32 AI060232-03/AI/NIAID NIH HHS
Reg. No./Substance:
0/Ligands; 0/Polymers; 0/Proteoglycans; 57-88-5/Cholesterol; 74-79-3/Arginine; 9002-98-6/Polyethyleneimine; 9007-49-2/DNA
Erratum In:
Biochim Biophys Acta. 2008 Oct;1783(10):2039

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