Document Detail


Cellular aspects of vascular remodeling in hypertension revealed by confocal microscopy.
MedLine Citation:
PMID:  9403567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular aspects of remodeling in intact arteries have not been fully investigated, mainly due to the lack of an appropriate methodology that allows for simple measurements. The aim of this study was to develop a method based on laser scanning confocal microscopy (LSCM), compare it with previous methodology, and apply it to the study of remodeling in hypertension. The morphology of mesenteric resistance arteries from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto rats (WKY) was determined with wire myography on one segment with a standardized diameter setting (0.9[d100]) and with perfusion myography on a second segment from the same artery at the calculated equivalent pressure. The second segments were stained with the nuclear dye Hoechst 33342 (live tissue) or propidium iodide (fixed tissue) and measured with LSCM and MetaMorph software. Compared with wire myography, perfusion myography showed similar differences from those previously reported. Compared with LSCM, perfusion myography showed a similar lumen but significantly smaller wall thickness in both live and fixed tissue, probably due to measurement underestimation. In the study with LSCM, arteries from SHRSP compared with those from WKY showed (1) reduced lumen, (2) altered cell density that was significantly increased in the adventitia, decreased in the media, and unchanged in the intima, (3) significantly increased medial volume, (4) significantly smaller endothelial cell nuclei, and (5) adventitial-like cells in the media. We conclude that (1) LSCM is a reliable and straightforward method to study morphology in intact vessels, (2) it provides new information on the cellular changes in remodeling, (3) adventitia might play an active role in the process of remodeling in hypertension, and (4) endothelium "remodels" in hypertension.
Authors:
S M Arribas; C Hillier; C González; S McGrory; A F Dominiczak; J C McGrath
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hypertension     Volume:  30     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-08     Completed Date:  1998-01-08     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1455-64     Citation Subset:  IM    
Affiliation:
Clinical Research Initiative in Heart Failure, Institute of Biomedical and Life Sciences, Western Infirmary, University of Glasgow, UK. S.Arribas@bio.gla.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrovascular Disorders
Electromyography / methods
Endothelium, Vascular / pathology,  physiology,  physiopathology
Female
Hypertension / pathology*,  physiopathology
Male
Mesenteric Arteries / pathology*,  physiology,  physiopathology
Microscopy, Confocal / methods
Muscle, Smooth, Vascular / pathology*,  physiology,  physiopathology
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Vascular Resistance
Grant Support
ID/Acronym/Agency:
//Wellcome Trust

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