Document Detail

Cellular alterations in cultured endothelial cells exposed to therapeutic ultrasound irradiation.
MedLine Citation:
PMID:  16162443     Owner:  NLM     Status:  MEDLINE    
Restoration of blood supply to tissue with impaired perfusion depends on spontaneous or mediated angiogenesis, which among other mechanisms includes stimulation, migration, and proliferation of endothelial cells (ECs). Therapeutic ultrasound (US) irradiation is known as an inducer of cellular modifications and is used to accelerate wound healing. An in vitro setup was developed in order to allow for a comprehensive investigation of cellular alterations induced in cultured ECs after exposure to different modes of therapeutic US irradiation. Viability assays revealed a higher rate of proliferation in the sonicated groups, although cell death was not observed. Visualization of actin stress fibers demonstrated partial disassembly of the fibers immediately after US sonication, with a maximum after about 2 h. However, 24 h following sonication the fibers regain normal appearance. A similar behavior was observed with the microtubules and focal adhesion complexes. Utilizing a wound healing assay revealed that migration rate of ECs is enhanced by US irradiation. These findings hint that therapeutic US sonication of ECs results in temporarily cellular alterations, which may induce tissue remodeling via stimulation of EC proliferation and migration.
Dalit Raz; Uri Zaretsky; Shmuel Einav; David Elad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endothelium : journal of endothelial cell research     Volume:  12     ISSN:  1062-3329     ISO Abbreviation:  Endothelium     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-09-15     Completed Date:  2005-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9412590     Medline TA:  Endothelium     Country:  United States    
Other Details:
Languages:  eng     Pagination:  201-13     Citation Subset:  IM    
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.
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MeSH Terms
Cell Proliferation
Cell Survival
Cells, Cultured
Endothelial Cells / cytology*
Focal Adhesions / chemistry*
Microtubules / chemistry*
Time Factors
Wound Healing

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