| Cellular uptake and photodynamic activity of protein nanocages containing methylene blue photosensitizing drug. | |
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MedLine Citation:
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PMID: 20132513 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also deliver photosensitizers to the inside of the target cells, protein nanocages provide a unique vehicle of biological origin for the intracellular delivery of photosensitizing molecules for PDT by protecting the photosensitizers from reactive biomolecules in the cell membranes, and yet providing a coherent, critical mass of destructive power (by way of singlet oxygen) upon specific light irradiation for photodynamic therapy of tumor cells. As a model, we demonstrated the successful encapsulation of methylene blue (MB) in apoferritin via a dissociation-reassembly process controlled by pH. The resulting MB-containing apoferritin nanocages show a positive effect on singlet oxygen production, and cytotoxic effects on MCF-7 human breast adenocarcinoma cells when irradiated at the appropriate wavelength (i.e. 633 nm). |
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Authors:
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Fei Yan; Yan Zhang; Kyu S Kim; Hsiang-Kuo Yuan; Tuan Vo-Dinh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-01-27 |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 86 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-06-16 Completed Date: 2010-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 662-6 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Duke University, Durham, NC, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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pathology,
therapy Cell Line, Tumor Drug Carriers / chemistry Female Humans Light Methylene Blue / administration & dosage* Nanoparticles / chemistry Photochemotherapy / methods* Photosensitizing Agents / administration & dosage* Proteins / pharmacokinetics, therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Photosensitizing Agents; 0/Proteins; 61-73-4/Methylene Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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