Document Detail


Cellular RNA synthesis in normal and mengovirus-infected L-929 cells.
MedLine Citation:
PMID:  201639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular RNA synthesis was studied in mouse L-929 cells and in these cells infected with mengovirus. RNA polymerases I, II, and III were partially purified and their chromatographic properties were analyzed by DEAE-Sephadex A-25 chromatography. RNA polymerase II was purified from mouse liver and its subunit structure was compared to that of normal and virus-infected L-929 cells by two-dimensional gel electrophoresis. By these criteria, the enzymes from all three sources were identical. The RNA synthetic activities and capacities of chromatins from normal and virus-infected cells were compared under a variety of conditions. The endogenous activity in chromatin from infected cells was inhibited relative to controls but the residual activity responded normally to stimulation by ammonium sulfate, heparin, and Sarkosyl. The template capacity of the chromatins was compared with added RNA polymerase II and by a rifampicin challenge assay utilizing Escherichia coli RNA polymerase. Identical results were obtained in each case. The number of growing RNA chains and the rates of their elongations were determined. The results showed that nuclei and chromatin from infected cells have a smaller number of RNA polymerase II molecules engaged in RNA synthesis than normal cells do but that the active molecules elongate RNA chains at the same rate.
Authors:
J W Apriletti; E E Penhoet
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  253     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-02-23     Completed Date:  1978-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  603-11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Transformation, Viral*
Chromatin
DNA-Directed RNA Polymerases / metabolism*
Kinetics
L Cells (Cell Line) / enzymology
Mengovirus*
RNA Polymerase I / isolation & purification,  metabolism
RNA Polymerase II / isolation & purification,  metabolism
RNA Polymerase III / isolation & purification,  metabolism
Templates, Genetic
Chemical
Reg. No./Substance:
0/Chromatin; EC 2.7.7.-/RNA Polymerase I; EC 2.7.7.-/RNA Polymerase II; EC 2.7.7.-/RNA Polymerase III; EC 2.7.7.6/DNA-Directed RNA Polymerases

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