Document Detail

Cellular Fatty Acids as Chemical Markers for Differentiation of Acinetobacter baumannii and Acinetobacter calcoaceticus.
MedLine Citation:
PMID:  23228842     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE: Gas chromatography (GC) was used to investigate the cellular fatty acid (CFA) composition of 141 Acinetobacter baumannii and 32 A. calcoaceticus isolates from different locations in China and to find chemical markers to differentiate these two closely related bacteria.
METHODS: Whole cell fatty acid methyl esters (FAMEs) were obtained by saponification, methylation, and extraction for GC analysis, followed by a standardized Microbial Identification System (MIS) analysis.
RESULTS: All A. baumannii and A. calcoaceticus strains contained some major fatty acids, namely, 18:1 ω9c, 16:0, Sum In Feature 3, 12:0, 17:1ω8c, 3-OH-12:0, 17:0, Sum In Feature 2, 2-OH-12:0, and 18:0 compounds. Although most of the total CFAs are similar between A. baumannii and A. calcoaceticus strains, the ratios of two pairs of CFAs, i.e., Sum In Feature 3/18:1 ω9c versus 16:0/18:1 ω9c and Sum In Feature 3/18:1 ω9c versus unknown 12.484/18:1 ω9c fatty acids, could differentiate these two closely related bacteria. A. baumannii could be easily classified into two subgroups by plotting some ratios such as Sum In Feature 3/16:0 versus 17:0 and Sum In Feature 3/2-OH-12:0 versus17:0 fatty acids.
CONCLUSION: The ratios of some CFAs could be used as chemical markers to distinguish A. baumannii from A. calcoaceticus.
Chao Yang; Zhao Biao Guo; Zong Min DU; Hui Ying Yang; Yu Jing Bi; Gui Qin Wang; Ya Fang Tan
Publication Detail:
Type:  Letter    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  25     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  711-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved.
Department of Clinical Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Beijing University of Chinese Medicine, Beijing 100029, China.
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