| Cellular Fatty Acid composition of stratified squamous epithelia after transplantation of ex vivo produced oral mucosa equivalent. | |
| | |
MedLine Citation:
|
PMID: 21937874 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Purpose: We have fabricated, for clinical application, artificial oral mucosa that totally excludes both heterogenic protein interaction and xenotransplantation. The purpose of this study is to compare the fatty acid composition of cell membrane phospholipids related to post-transplantation epithelial regeneration. Materials and Methods: Cultured keratinocytes, keratinocytes at 2, 3, 4, and 9 weeks after transplantation, and normal oral keratinocytes were compared by gas chromatography for the composition of 23 fatty acids. The relation between the composition of cell membrane fatty acids, and the glucose metabolism was immunohistochemically analyzed. Results: 1. Even after transplantation, cultured keratinocytes retained the same ratio of palmitic acid as that of normal oral keratinocytes. 2. Essential fatty acids decreased markedly in cultured keratinocyte membranes to the same composition as that of normal oral mucosa 2 weeks after transplantation. 3. The percent composition of palmitoleic acid in cultured keratinocytes was significantly higher than that in post-transplanted keratinocytes; it decreased 2 weeks after artificial mucosa transplantation, but became similar to that in 3 weeks thereafter. 4. The entire population of stratified keratinocytes in EVPOME before transplantation expressed GLUT-1 protein. Conclusion: Our findings suggest that post-artificial mucosa epithelialisation allows keratinocytes to proliferate while consuming palmitic acid, and then diet-provided essential fatty acids induce the keratinocytes to differentiate. Complete clinical epithelialisation of the transplant wound requires 4 weeks; however, within 3 weeks of transplantation, cultured cells of a specific metabolic mechanism change into or are replaced by keratinocytes of a normal metabolic mechanism similar to that of surrounding tissue. |
| | |
Authors:
|
Kentaro Moriyama; Satoshi Yokoo; Hiroto Terashi; Takahide Komori |
Related Documents
:
|
20362044 - Homogeneous, heterogeneous and enzymatic catalysis for transesterification of high free... 20412234 - Brazilian oils and butters: the effect of different fatty acid chain composition on hum... 17616134 - Statistical characterization of sicilian olive oils from the peloritana and maghrebian ... 11829634 - Stability of virgin olive oil. 1. autoxidation studies. 1856604 - Higher aliphatic 2,4-diketones: a ubiquitous lipid class with chelating properties, in ... 3949234 - Effect of small doses of deoxycholic acid on bile cholesterol saturation in patients wi... |
Publication Detail:
|
Type: Journal Article Date: 2011-05-11 |
Journal Detail:
|
Title: The Kobe journal of medical sciences Volume: 56 ISSN: 1883-0498 ISO Abbreviation: Kobe J Med Sci Publication Date: 2011 |
Date Detail:
|
Created Date: 2011-09-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0413531 Medline TA: Kobe J Med Sci Country: Japan |
Other Details:
|
Languages: eng Pagination: E253-62 Citation Subset: IM |
Affiliation:
|
Department of Oral and Maxillofacial Surgery Kobe University Graduate School of Medicine, Kobe, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of Ultrasound Irradiation on ?-SMA and TGF-?1 Expression in Human Dermal Fibroblasts.
Next Document: Assessment of Dissociation Symptoms in Patients with Mental Disorders by the Dissociation Questionna...