| Cellular evaluation of synthesized insulin/transferrin bioconjugates for oral insulin delivery using intelligent complexation hydrogels. | |
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MedLine Citation:
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PMID: 20034125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insulin transport across the epithelial cell layer in the small intestine was studied using insulin/transferrin conjugates with and without the presence of P(MAA-g-EG) microparticles in contact with a co-culture of Caco-2/HT29-MTX cells. The insulin/transferrin conjugate was shown to increase transport relative to pure insulin by a factor of 7, achieving an apparent permeability of 37 x 10(9) cm . s(-1). The presence of P(MAA-g-EG) microparticles increased conjugate transport by a factor of 14 times relative to insulin, achieving an apparent permeability of 72.8 x 10(9) cm . s(-1). The presence of the microparticles in solution was found to improve conjugate transport by nearly 100% with little to no change in cell monolayer integrity. |
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Authors:
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Justin P Shofner; Margaret A Phillips; Nicholas A Peppas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Macromolecular bioscience Volume: 10 ISSN: 1616-5195 ISO Abbreviation: Macromol Biosci Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-09-13 Revised Date: 2012-01-11 |
Medline Journal Info:
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Nlm Unique ID: 101135941 Medline TA: Macromol Biosci Country: Germany |
Other Details:
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Languages: eng Pagination: 299-306 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Caco-2 Cells Cell Membrane Permeability Cell-Derived Microparticles / metabolism Coculture Techniques Drug Delivery Systems* Electric Impedance Epithelial Cells / metabolism HT29 Cells Humans Hydrogels / chemistry* Insulin / administration & dosage*, metabolism* Polyethylene Glycols / chemistry Polymethacrylic Acids / chemistry Protein Transport Solutions Transferrin / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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EB-000246/EB/NIBIB NIH HHS; R01 EB000246-17A2/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hydrogels; 0/Insulin; 0/Polyethylene Glycols; 0/Polymethacrylic Acids; 0/Solutions; 0/Transferrin; 25087-26-7/polymethacrylic acid |
| Comments/Corrections | |
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