| Cellular changes in diabetic and idiopathic gastroparesis. | |
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MedLine Citation:
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PMID: 21300066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Cellular changes associated with diabetic and idiopathic gastroparesis are not well described. The aim of this study was to describe histologic abnormalities in gastroparesis and compare findings in idiopathic versus diabetic gastroparesis. METHODS: Full-thickness gastric body biopsy specimens were obtained from 40 patients with gastroparesis (20 diabetic) and matched controls. Sections were stained for H&E and trichrome and immunolabeled with antibodies against protein gene product (PGP) 9.5, neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide, substance P, and tyrosine hydroxylase to quantify nerves, S100β for glia, Kit for interstitial cells of Cajal (ICC), CD45 and CD68 for immune cells, and smoothelin for smooth muscle cells. Tissue was also examined by transmission electron microscopy. RESULTS: Histologic abnormalities were found in 83% of patients. The most common defects were loss of ICC with remaining ICC showing injury, an abnormal immune infiltrate containing macrophages, and decreased nerve fibers. On light microscopy, no significant differences were found between diabetic and idiopathic gastroparesis with the exception of nNOS expression, which was decreased in more patients with idiopathic gastroparesis (40%) compared with diabetic patients (20%) by visual grading. On electron microscopy, a markedly increased connective tissue stroma was present in both disorders. CONCLUSIONS: This study suggests that on full-thickness biopsy specimens, cellular abnormalities are found in the majority of patients with gastroparesis. The most common findings were loss of Kit expression, suggesting loss of ICC, and an increase in CD45 and CD68 immunoreactivity. These findings suggest that examination of tissue can lead to valuable insights into the pathophysiology of these disorders and offer hope that new therapeutic targets can be found. |
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Authors:
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Madhusudan Grover; Gianrico Farrugia; Matthew S Lurken; Cheryl E Bernard; Maria Simonetta Faussone-Pellegrini; Thomas C Smyrk; Henry P Parkman; Thomas L Abell; William J Snape; William L Hasler; Aynur Ünalp-Arida; Linda Nguyen; Kenneth L Koch; Jorges Calles; Linda Lee; James Tonascia; Frank A Hamilton; Pankaj J Pasricha; |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2011-02-04 |
Journal Detail:
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Title: Gastroenterology Volume: 140 ISSN: 1528-0012 ISO Abbreviation: Gastroenterology Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-25 Completed Date: 2011-06-28 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 1575-85.e8 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2011 AGA Institute. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Mayo Clinic, Rochester, Minnesota, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Biopsy Diabetes Mellitus / pathology* Female Gastric Emptying / physiology* Gastroparesis / pathology*, physiopathology Humans Interstitial Cells of Cajal / ultrastructure Male Microscopy, Electron, Transmission Middle Aged Stomach / pathology*, physiopathology Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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DK57061/DK/NIDDK NIH HHS; P01 DK068055-05S10001/DK/NIDDK NIH HHS; P01 DK068055-06A1/DK/NIDDK NIH HHS; P01 DK68055/DK/NIDDK NIH HHS; P30 DK084567-02/DK/NIDDK NIH HHS; R01 DK057061-12/DK/NIDDK NIH HHS; U01 DK073974-05/DK/NIDDK NIH HHS; U01 DK073975-05/DK/NIDDK NIH HHS; U01 DK073983-05/DK/NIDDK NIH HHS; U01 DK073985-05/DK/NIDDK NIH HHS; U01 DK074007-05/DK/NIDDK NIH HHS; U01 DK074008-05/DK/NIDDK NIH HHS; U01DK073974/DK/NIDDK NIH HHS; U01DK073975/DK/NIDDK NIH HHS; U01DK073983/DK/NIDDK NIH HHS; U01DK073985/DK/NIDDK NIH HHS; U01DK074007/DK/NIDDK NIH HHS; U01DK074008/DK/NIDDK NIH HHS |
| Investigator | |
Investigator/Affiliation:
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Pankaj Jay Pasricha / ; Linda Nguyen / ; Nighat Ullah / ; William Snape / ; Robin Bishop / ; Nata DeVole / ; Mary Greene / ; Sue Louiseau / ; Amy Marincek / ; Shelly Parker / ; Eve Pillor / ; Courtney Ponsetto / ; Katerina Shetler / ; Gianrico Farrugia / ; Madhusudan Grover / ; Cheryl Bernard / ; Matt Lurken / ; K Robert Shen / ; Michael Sarr / ; Michael Kendrick / ; Henry P Parkman / ; Siva Doma / ; Javier Gomez / ; Steven Kantor / ; Vanessa Lytes / ; Amiya Palit / ; Zeeshan Ramzan / ; Priyanka Sachdeva / ; Kellie Simmons / ; Sean Harbison / ; Richard W McCallum / ; Reza Hejazi / ; Irene Sarosiek / ; Denise Vasquez / ; Natalia Vega / ; William Hasler / ; Michelle Atkinson / ; Radoslav Coleski / ; Thomas Abell / ; JoAnne Fordham / ; Olivia Henry / ; Archana Kedar / ; Valerie McNair / ; Susanne Pruett / ; Margaret Smith / ; Danielle Spree / ; Kenneth Koch / ; Lynn Baxter / ; Jorge Calles / ; Samantha Culler / ; Judy Hooker / ; Frank Hamilton / ; Steven James / ; Rebecca Torrance / ; Rebekah Van Raaphorst / ; James Tonascia / ; Patricia Belt / ; Ryan Colvin / ; Michele Donithan / ; Mika Green / ; Milana Isaacson / ; Wana Kim / ; Linda Lee / ; Alison Lydecker / ; Pamela Mann / ; Laura Miriel / ; Alice Sternberg / ; Aynur Ünalp-Arida / ; Mark Van Natta / ; Ivana Vaughn / ; Laura Wilson / ; Katherine Yates / |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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