Document Detail

The Cellular Behavior and SEM Evaluation of ProRoot and Root MTAs on Fibroblast L929.
MedLine Citation:
PMID:  24454449     Owner:  NLM     Status:  PubMed-not-MEDLINE    
INTRODUCTION: Mineral trioxide aggregate is being widely used for root end filling, pulp capping, perforation repair, and other endodontic procedures. Recently, a material similar to ProRoot mineral trioxide aggregate (PMTA) was developed in Iran named Root mineral trioxide aggregate (RMTA) with the claim of having the exact result of original MTA. The purpose of this study was to compare the amount of cell cytotoxicity of RMTA with PMTA at three different time periods using scanning electron microscopy (SEM) as well as the amount of cell viability at the above mentioned period.
MATERIALS AND METHODS: Three culture plates in each group were packed with a homogenous layer of PMTA and RMTA prepared according to manufacturers instruction. A plate of media without any material was used as control in each group. The material set for 72 h in CO2 incubator and 2x10 of fibroblast L929 was added to each plate. SEM evaluation with x800-3000 magnification and cell viability counting using trepan blue counting method were done after 48, 72, and 168 hours.
RESULTS: There was no significant difference between cell viability of PMTA and RMTA, although the amount of cells remained viable in PMTA group was higher at 48 and 168 hours while for RMTA, it was higher after 72 hours. The SEM evaluation showed that PMTA compared with RMTA has less porosity, but relatively similar amount of cell coverage was detected for both materials after 168 hours.
CONCLUSION: ProRoot and Root MTAs showed comparative biocompatibility while evaluated in vitro. The results suggest that RMTA can be used as an alternative for PMTA in clinical trials.
Fariborz Moazami; Samira Shahsiah
Related Documents :
24856959 - Force-controlled manipulation of single cells: from afm to fluidfm.
23913869 - Impact of direct cell co-cultures on human adipose-derived stromal cells and nucleus pu...
23804489 - Crf type 2 receptors mediate the metabolic effects of ghrelin in c2c12 cells.
24885389 - Characterization of multi-drug tolerant persister cells in streptococcus suis.
23868059 - H-ras transfers from b to t cells via tunneling nanotubes.
24705439 - Predictive models of type 1 diabetes progression: understanding t-cell cycles and their...
21438179 - Effect of constitutively active ras overexpression on cell growth in recombinant chines...
21496299 - Depletion of the histone chaperone tnasp inhibits proliferation and induces apoptosis i...
15736399 - Visualizing portal vein metastatic trafficking to the liver with green fluorescent prot...
Publication Detail:
Type:  Journal Article     Date:  2006-10-01
Journal Detail:
Title:  Iranian endodontic journal     Volume:  1     ISSN:  1735-7497     ISO Abbreviation:  Iran Endod J     Publication Date:  2006  
Date Detail:
Created Date:  2014-01-23     Completed Date:  2014-01-23     Revised Date:  2014-01-30    
Medline Journal Info:
Nlm Unique ID:  101497347     Medline TA:  Iran Endod J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effect of different suture removal time intervals on surgical wound healing.
Next Document:  Bacterial leakage of mineral trioxide aggregates and portland cement.