| Cellular Assay Optimization: Part I: The Use of Large-Scale Transiently Transfected Cryobanks and Introduction of a c-Myc Tag to Design a Standardized ELISA Process. | |
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MedLine Citation:
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PMID: 21841143 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study investigated the use of large-scale transiently transfected cryopreserved cells for medium-throughput cellular screening. The data generated indicated that preprepared transiently transfected cryobanks can be used for cell-based assays and in fact can greatly enhance the consistency of data generated by cellular screens. in addition to this, a generic enzyme-linked immunosorbent assay method was designed that introduced a c-Myc tag to four different targets and allowed all four cell assays to be run using a standardized process. These process improvements yielded cost savings and greatly reduced the required resource, as well as reducing timelines for developing cellular assays. |
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Authors:
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Marie A Macmillan; David I Fisher; Karen Roberts; Jonathan P Orme |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-12 |
Journal Detail:
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Title: Journal of biomolecular screening Volume: - ISSN: 1552-454X ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9612112 Medline TA: J Biomol Screen Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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