Document Detail

Cellular activity of bioactive nanograined/ultrafine-grained materials.
MedLine Citation:
PMID:  20045089     Owner:  NLM     Status:  MEDLINE    
Our recent electron microscopy study on biomimetic nanostructured coatings on nanograined/ultrafine-grained (NG/UFG) substrates [Mater Sci Eng C 2009;29:2417-27] indicated that electrocrystallized nanohydroxyapatite (nHA) on phase-reversion-induced NG/UFG substrates exhibited a vein-type interconnected and fibrillar structure that closely mimicked the hierarchical structure of bone. The fibrillar structure on NG/UFG substrate is expected to be more favorable for cellular response than a planar surface. In contrast, hydroxyapatite (HA) coating on coarse-grained (CG) substrate more closely resembled a film rather than a fibrillar structure. Inspired by the differences in the structure of HA coating, we describe here the cell-substrate interactions of pre-osteoblasts (MC 3T3-E1) on bioactive NG/UFG and CG austenitic stainless steel substrates. NG/UFG austenitic stainless steel was obtained by a novel controlled phase-reversion annealing of cold-deformed austenite. This example provides an illustration of how a combination of cellular and molecular biology, materials science and engineering can advance our understanding of cell-substrate interactions. Interestingly, the cellular response of nanohydroxyapatite (nHA)-coated NG/UFG substrate demonstrated superior cytocompatibility, improved initial cell attachment, higher viability and proliferation, and well-spread morphology in relation to HA-coated CG substrate and their respective uncoated (bare) counterparts as implied by fluorescence and electron microscopy and MTT assay. Similar conclusions were derived from an immunofluorescence study that involved examination of the expression levels of vinculin focal adhesion contacts associated with dense actin stress fibers and fibronectin, protein analysis through protein bands in SDS-PAGE, and quantitative total protein assay. The enhancement of cellular response followed the sequence: nHA-coated NG/UFG>nHA-coated CG>NG/UFG>CG substrates. The outcomes of the study are expected to counter the challenges associated with the engineering of nanostructured surfaces with specific physical and surface properties for medical devices with significantly improved cellular response.
R D K Misra; W W Thein-Han; S A Mali; M C Somani; L P Karjalainen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-01-04
Journal Detail:
Title:  Acta biomaterialia     Volume:  6     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-08-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  2826-35     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Center for Structural and Functional Materials, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
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MeSH Terms
3T3 Cells
Cell Adhesion
Cell Proliferation
Durapatite / chemistry
Electrophoresis, Polyacrylamide Gel
Microscopy, Fluorescence
Reg. No./Substance:

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