Document Detail

Cells from an anhydrobiotic chironomid survive almost complete desiccation.
MedLine Citation:
PMID:  19850023     Owner:  NLM     Status:  MEDLINE    
Dry-preservation of nucleated cells from multicellular animals represents a significant challenge in life science. As anhydrobionts can tolerate a desiccated state, their cells and organs are expected to show high desiccation tolerance in vitro. In the present study, we established cell lines derived from embryonic tissues of an anhydrobiotic chironomid, Polypedilum vanderplanki, designated as Pv11 and Pv210. Salinity stress induced the expression of a set of anhydrobiosis-related genes in both Pv11 and Pv210 cells, suggesting that at least a part of cells can autonomously control the physiological changes for the entry into anhydrobiosis. When desiccated with medium supplemented with 300 mM trehalose or sucrose and stored for 4 weeks in dry air (approximately 5% relative humidity), a small percentage of the cells was found to be viable upon rehydration, although surviving cells seemed not to be able to multiply. We also attempted dry-preservation of organs isolated from P. vanderplanki larvae, and found that a proportion of cells in some organs, including fat body, testis, nerve and dorsal vessel, tolerated in vitro desiccation.
Yuichi Nakahara; Shigeo Imanishi; Kanako Mitsumasu; Yasushi Kanamori; Ken-ichi Iwata; Masahiko Watanabe; Takahiro Kikawada; Takashi Okuda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-20
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  138-46     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Anhydrobiosis Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
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MeSH Terms
Base Sequence
Cell Line
Cell Survival
Chironomidae / cytology*,  embryology,  genetics
DNA Primers / genetics
Desiccation / methods*
Gene Expression
Genes, Insect
Larva / anatomy & histology
Organ Preservation / methods
Osmotic Pressure
Preservation, Biological / methods*
Stress, Physiological
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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