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Cells dispersed from Marinobacter hydrocarbonoclasticus SP17 biofilm exhibit a specific protein profile associated with a higher ability to reinitiate biofilm development at the hexadecane-water interface.
MedLine Citation:
PMID:  21087383     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Biofilm formation by marine hydrocarbonoclastic bacteria is commonly observed and has been recognized as an important mechanism for the biodegradation of hydrocarbons. In order to colonize new oil-water interfaces, surface-attached communities of hydrocarbonoclastic bacteria must release cells into the environment. Here we explored the physiology of cells freshly dispersed from a biofilm of Marinobacter hydrocarbonoclasticus developing at the hexadecane-water interface, by combining proteomic and physiological approaches. The comparison of the dispersed cells' proteome with those of biofilm, logarithmic- and stationary-phase planktonic cells indicated that dispersed cells had lost most of the biofilm phenotype and expressed a specific proteome. Two proteins involved in cell envelope maturation, DsbA and CtpA, were exclusively detected in dispersed cells, suggesting a reshaping of the cell envelopes during biofilm dispersal. Furthermore, dispersed cells exhibited a higher affinity for hexadecane and initiated more rapidly biofilm formation on hexadecane than the reference planktonic cells. Interestingly, storage wax esters were rapidly degraded in dispersed cells, suggesting that their observed physiological properties may rely on reserve mobilization. Thus, by promoting oil surface colonization, cells emigrating from the biofilm could contribute to the success of marine hydrocarbonoclastic bacteria in polluted environments.
Authors:
Pierre-Joseph Vaysse; Pierre Sivadon; Philippe Goulas; Régis Grimaud
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Publication Detail:
Type:  Journal Article     Date:  2010-11-18
Journal Detail:
Title:  Environmental microbiology     Volume:  13     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  737-46     Citation Subset:  IM    
Copyright Information:
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, Equipe Environnement et Microbiologie, UMR5254 CNRS, IBEAS, Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
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