Document Detail


Cells derived from regenerated endothelium of the porcine coronary artery contain more oxidized forms of apolipoprotein-B-100 without a modification in the uptake of oxidized LDL.
MedLine Citation:
PMID:  12904688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increased accumulation of lipoproteins and cholesterol within cells from regenerated endothelium may be responsible for their reported dysfunction. This study compared the presence and uptake of oxidized forms of low-density lipoprotein (LDL) in cells derived from native and regenerated endothelium. Four weeks after balloon denudation, primary cultures of native and regenerated endothelial cells were prepared from porcine coronary arteries. Regenerated endothelium stained more strongly using an antibody against oxidized lipoproteins. The increase in oxidized forms of apolipoprotein-B-100 exhibited by cells from regenerated endothelium was not due to an increase in extracellular-induced oxidation of native LDL, measured as the production of thiobarbituric-acid-reactive substances, being identical in both cell types. Intracellular cholesterol and cholesterol ester content were unchanged in regenerated cells. Using flow cytometry, accumulation of oxidized LDL was investigated further by quantifying the uptake of a mildly oxidized preparation of 1,1'-dioctadecyl-3,3,3',3-tetramethyl-indocarbocyanine perchlorate-labelled LDL. The parameters of uptake, EC(50) and E(max), were not different between cells from native and regenerated endothelium suggesting that the number of LOX-1 receptors was identical in the two cell types. Moreover, a negative correlation between the increased uptake of acetylated LDL and decreased cGMP production in response to bradykinin was observed in cells from regenerated endothelium. Thus, the increased incorporation of modified LDL and their intracellular oxidation could be responsible for the alteration in NO production. The presence of oxidized forms of LDL may be a marker of endothelium regeneration and could be involved in the endothelial dysfunction of pig coronary arteries 4 weeks after balloon denudation.
Authors:
Simon Kennedy; Marie-Pierre Fournet-Bourguignon; Christine Breugnot; Maria Castedo-Delrieu; Ludovic Lesage; Hélène Reure; Cyril Briant; Stephane Leonce; Jean-Paul Vilaine; Paul M Vanhoutte
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Publication Detail:
Type:  Journal Article     Date:  2003-08-06
Journal Detail:
Title:  Journal of vascular research     Volume:  40     ISSN:  1018-1172     ISO Abbreviation:  J. Vasc. Res.     Publication Date:    2003 Jul-Aug
Date Detail:
Created Date:  2003-09-23     Completed Date:  2003-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  389-98     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Affiliation:
Institut de Recherches Servier, Suresnes, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoprotein B-100
Apolipoproteins B / metabolism*
Carbocyanines
Cholesterol / metabolism
Cholesterol, LDL / pharmacokinetics
Copper Sulfate / pharmacology
Coronary Vessels / cytology,  drug effects,  metabolism*
Cyclic GMP / metabolism
Endothelium, Vascular / cytology,  drug effects,  metabolism*
Female
Fluorescent Dyes
Immunohistochemistry
Lipoproteins, LDL / metabolism*
Male
Oxidation-Reduction
Regeneration*
Swine
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Apolipoprotein B-100; 0/Apolipoproteins B; 0/Carbocyanines; 0/Cholesterol, LDL; 0/Fluorescent Dyes; 0/Lipoproteins, LDL; 0/Thiobarbituric Acid Reactive Substances; 0/oxidized low density lipoprotein; 40957-95-7/3,3'-dioctadecylindocarbocyanine; 57-88-5/Cholesterol; 7665-99-8/Cyclic GMP; 7758-98-7/Copper Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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