Document Detail


Cells on a chip--the use of electric properties for highly sensitive monitoring of blood-derived factors involved in angiotensin II type 1 receptor signalling.
MedLine Citation:
PMID:  16121033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We developed a highly sensitive cardiomyocyte based screening system for the non-destructive electronic detection of chronotropic drugs and tissue-secreted factors involved in AT1 receptor-mediated cardiovascular diseases. METHODS: For this purpose we cultured spontaneously beating neonatal rat cardiomyocytes on microelectrode arrays (MEAs), and tested the optimised, stable culture parameters for a reproducible real-time recording of alterations in contraction frequency. After the evaluation of culture parameters, computer-based electronic measurement systems were used for counting of contractions by recording of the field potential of cardiomyocytes. RESULTS: Using the biosensor, angiotensin II, the predominant ligand of the AT1 receptor, was detected at very low concentrations of 10(-11) M via altered contractions of cardiomyocytes. Moreover, we demonstrated that cardiomyocyte coupled microarrays allow the detection of blood-derived low concentrated anti-AT1 receptor autoimmune antibodies of pregnant women suffering from preeclampsia. CONCLUSION: This study demonstrates the first well-suited electrophysiological recording of cardiomyocytes on multielectrode arrays as a benefit for functional biomonitoring for the detection of AT1 receptor/ligand interactions and other marker proteins in sera directed to cardiovascular diseases.
Authors:
Andrée Rothermel; Randy Kurz; Markus Rüffer; Winnie Weigel; Heinz-Georg Jahnke; Anna K Sedello; Holger Stepan; Renaldo Faber; Kai Schulze-Forster; Andrea A Robitzki
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  16     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-25     Completed Date:  2005-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  51-8     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 S. Karger AG, Basel.
Affiliation:
Center for Biotechnology and Biomedicine, Department of Molecular biological- biochemical Processing Technology, University of Leipzig, Germany. andree.rothermel@bbz.uni-leipzig.de
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MeSH Terms
Descriptor/Qualifier:
Angiotensin II / analysis
Animals
Animals, Newborn
Autoantibodies / blood
Base Sequence
Biosensing Techniques / methods*,  statistics & numerical data
Cells, Immobilized*
Electrophysiology
Female
Humans
Myocytes, Cardiac / metabolism*
Pre-Eclampsia / immunology
Pregnancy
RNA, Messenger / genetics
Rats
Receptor, Angiotensin, Type 1 / genetics,  immunology,  metabolism*
Reproducibility of Results
Sensitivity and Specificity
Signal Transduction
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 11128-99-7/Angiotensin II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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