| Cells on a chip--the use of electric properties for highly sensitive monitoring of blood-derived factors involved in angiotensin II type 1 receptor signalling. | |
| | |
MedLine Citation:
|
PMID: 16121033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: We developed a highly sensitive cardiomyocyte based screening system for the non-destructive electronic detection of chronotropic drugs and tissue-secreted factors involved in AT1 receptor-mediated cardiovascular diseases. METHODS: For this purpose we cultured spontaneously beating neonatal rat cardiomyocytes on microelectrode arrays (MEAs), and tested the optimised, stable culture parameters for a reproducible real-time recording of alterations in contraction frequency. After the evaluation of culture parameters, computer-based electronic measurement systems were used for counting of contractions by recording of the field potential of cardiomyocytes. RESULTS: Using the biosensor, angiotensin II, the predominant ligand of the AT1 receptor, was detected at very low concentrations of 10(-11) M via altered contractions of cardiomyocytes. Moreover, we demonstrated that cardiomyocyte coupled microarrays allow the detection of blood-derived low concentrated anti-AT1 receptor autoimmune antibodies of pregnant women suffering from preeclampsia. CONCLUSION: This study demonstrates the first well-suited electrophysiological recording of cardiomyocytes on multielectrode arrays as a benefit for functional biomonitoring for the detection of AT1 receptor/ligand interactions and other marker proteins in sera directed to cardiovascular diseases. |
| | |
Authors:
|
Andrée Rothermel; Randy Kurz; Markus Rüffer; Winnie Weigel; Heinz-Georg Jahnke; Anna K Sedello; Holger Stepan; Renaldo Faber; Kai Schulze-Forster; Andrea A Robitzki |
Related Documents
:
|
11563933 - Changes in angiotensin at1 receptor mrna levels in the rat brain after immobilization s... 11146423 - Cloning, pharmacological characterization and brain distribution of the rat apelin rece... 16449733 - Preventive effect of erythropoietin on cardiac dysfunction in doxorubicin-induced cardi... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 16 ISSN: 1015-8987 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2005 |
Date Detail:
|
Created Date: 2005-08-25 Completed Date: 2005-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 51-8 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2005 S. Karger AG, Basel. |
Affiliation:
|
Center for Biotechnology and Biomedicine, Department of Molecular biological- biochemical Processing Technology, University of Leipzig, Germany. andree.rothermel@bbz.uni-leipzig.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiotensin II
/
analysis Animals Animals, Newborn Autoantibodies / blood Base Sequence Biosensing Techniques / methods*, statistics & numerical data Cells, Immobilized* Electrophysiology Female Humans Myocytes, Cardiac / metabolism* Pre-Eclampsia / immunology Pregnancy RNA, Messenger / genetics Rats Receptor, Angiotensin, Type 1 / genetics, immunology, metabolism* Reproducibility of Results Sensitivity and Specificity Signal Transduction |
| Chemical | |
Reg. No./Substance:
|
0/Autoantibodies; 0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 11128-99-7/Angiotensin II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Modulation of H+ transport mechanisms by interleukin-1 in isolated bovine articular chondrocytes.
Next Document: Different signaling pathways involved in glucose- and cell swelling-induced insulin secretion by rat...