Document Detail


Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay.
MedLine Citation:
PMID:  22347388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the process of cancer cell invasion through basement membrane or in the stroma. We have extended the characterization of the circular invasion assay and found that it provides a simple and amenable system to study cell invasion in matrix in an environment that closely mimics 3D invasion. Furthermore, it allows detailed microscopic analysis of both live and fixed cells during the invasion process. We find that cells invade in a protease dependent manner in this assay and that they assemble focal adhesions and invadopodia that resemble structures visualized in 3D embedded cells. We propose that this is a useful assay for routine and medium throughput analysis of invasion of cancer cells in vitro and the study of cells migrating in a 3D environment.
Authors:
Xinzi Yu; Laura M Machesky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-08
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-07-31     Revised Date:  2014-10-07    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e30605     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Basement Membrane
Cell Adhesion
Collagen
Drug Combinations
Humans
Laminin
Metalloproteases / physiology*
Models, Biological*
Neoplasm Invasiveness / pathology*
Proteoglycans
Grant Support
ID/Acronym/Agency:
15673//Cancer Research UK; G117/569//Medical Research Council; //Cancer Research UK
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 119978-18-6/matrigel; 9007-34-5/Collagen; EC 3.4.-/Metalloproteases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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