Document Detail

Cells Uptake and Recover from Protein Stabilized Single Wall Carbon Nanotubes with Two Distinct Rates.
MedLine Citation:
PMID:  22458848     Owner:  NLM     Status:  Publisher    
Single wall carbon nanotubes (SWCNTs) are increasingly being investigated for use in biomedical applications for intra-cellular imaging and ablation, as well as vehicles for drug and gene delivery. One major obstacle to the development of safe, controlled and effective SWCNT-based biomedical materials is limited quantification of dosage- and time-dependent uptake kinetics, cellular effects and recovery profiles. Here, we quantified NIH-3T3 cellular uptake of and recovery from individualized SWCNTs dispersed using a biocompatible dispersing agent, bovine serum albumin (BSA). Uptake and recovery were determined by monitoring the mass of SWCNTs-BSA per cell, as a function of SWCNTs-BSA over the concentration range of 1 to 100 μg mL-1 and time range of seconds to days. To determine SWCNTs-BSA biocompatibility as a function of uptake and recovery, cytotoxicity, proliferation potential and cell phenotype were monitored for each condition. Interestingly, the rate of cellular uptake of SWCNTs-BSA was rapid, reaching steady state within ~1 min, in agreement with modeling. We also observed a threshold SWCNT exposure level (> 1 μg mL-1) above which internalization is saturated and uptake scales linearly with exposure amount. Cells were able to recover from SWCNTs-BSA over ~30 hr, regardless of dosage level or exposure time. We suggest that these differential rates of uptake and recovery, quantified in our work, may enable cell-based SWCNT delivery systems.
Brian D Holt; Kris Noel Dahl; Mohammad F Islam
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-29
Journal Detail:
Title:  ACS nano     Volume:  -     ISSN:  1936-086X     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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