| Cells Uptake and Recover from Protein Stabilized Single Wall Carbon Nanotubes with Two Distinct Rates. | |
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MedLine Citation:
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PMID: 22458848 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Single wall carbon nanotubes (SWCNTs) are increasingly being investigated for use in biomedical applications for intra-cellular imaging and ablation, as well as vehicles for drug and gene delivery. One major obstacle to the development of safe, controlled and effective SWCNT-based biomedical materials is limited quantification of dosage- and time-dependent uptake kinetics, cellular effects and recovery profiles. Here, we quantified NIH-3T3 cellular uptake of and recovery from individualized SWCNTs dispersed using a biocompatible dispersing agent, bovine serum albumin (BSA). Uptake and recovery were determined by monitoring the mass of SWCNTs-BSA per cell, as a function of SWCNTs-BSA over the concentration range of 1 to 100 μg mL-1 and time range of seconds to days. To determine SWCNTs-BSA biocompatibility as a function of uptake and recovery, cytotoxicity, proliferation potential and cell phenotype were monitored for each condition. Interestingly, the rate of cellular uptake of SWCNTs-BSA was rapid, reaching steady state within ~1 min, in agreement with modeling. We also observed a threshold SWCNT exposure level (> 1 μg mL-1) above which internalization is saturated and uptake scales linearly with exposure amount. Cells were able to recover from SWCNTs-BSA over ~30 hr, regardless of dosage level or exposure time. We suggest that these differential rates of uptake and recovery, quantified in our work, may enable cell-based SWCNT delivery systems. |
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Authors:
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Brian D Holt; Kris Noel Dahl; Mohammad F Islam |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-29 |
Journal Detail:
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Title: ACS nano Volume: - ISSN: 1936-086X ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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