Document Detail

Cells Transformed by PLC-Gamma 1 Overexpression are Highly Sensitive to Clostridium difficile Toxin A-Induced Apoptosis and Mitotic Inhibition.
MedLine Citation:
PMID:  22297219     Owner:  NLM     Status:  In-Data-Review    
Phospholipase C-gammal (PLC-gammal) expression is associated with cellular transformation. Notably, PLC-gamma is up-regulated in colorectal cancer tissue and breast carcinoma. Because exotoxins released by Clostridium botulinum have been shown to induce apoptosis and promote growth arrest in various cancer cell lines, we examined here the potential of Clostridium difficile toxin A to selectively induce apoptosis in cells transformed by PLC-gammal overexpression. We found that PLC-gammal-transformed cells, but not vectortransformed (control) cells, were highly sensitive to C. difficile toxin A-induced apoptosis and mitotic inhibition. Moreover, expression of the proapoptotic Bcl2 family member, Bim, and activation of caspase-3 were significantly up-regulated by toxin A in PLC-gammal-transformed cells. Toxin A-induced cell rounding and paxillin dephosphorylation were also significantly higher in PLC-gammal-transformed cells than in control cells. These findings suggest that C. difficile toxin A may have potential as an anticancer agent against colorectal cancers and breast carcinomas in which PLC-gammal is highly up-regulated.
Hyo Jung Nam; Jin Ku Kang; Jong Soo Chang; Min Soo Lee; Seung Taek Nam; Hyun Woo Jung; Sung-Kuk Kim; Eun-Mi Ha; Heon Seok; Seung Woo Son; Young Joo Park; Ho Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microbiology and biotechnology     Volume:  22     ISSN:  1738-8872     ISO Abbreviation:  J. Microbiol. Biotechnol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431852     Medline TA:  J Microbiol Biotechnol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  50-7     Citation Subset:  IM    
Department of Life Science, College of Natural Science, Daejin University, Pochen, Korea.
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