| Cell wall peptidoglycan architecture in Bacillus subtilis. | |
| | |
MedLine Citation:
|
PMID: 18784364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The bacterial cell wall is essential for viability and shape determination. Cell wall structural dynamics allowing growth and division, while maintaining integrity is a basic problem governing the life of bacteria. The polymer peptidoglycan is the main structural component for most bacteria and is made up of glycan strands that are cross-linked by peptide side chains. Despite study and speculation over many years, peptidoglycan architecture has remained largely elusive. Here, we show that the model rod-shaped bacterium Bacillus subtilis has glycan strands up to 5 microm, longer than the cell itself and 50 times longer than previously proposed. Atomic force microscopy revealed the glycan strands to be part of a peptidoglycan architecture allowing cell growth and division. The inner surface of the cell wall has a regular macrostructure with approximately 50 nm-wide peptidoglycan cables [average 53 +/- 12 nm (n = 91)] running basically across the short axis of the cell. Cross striations with an average periodicity of 25 +/- 9 nm (n = 96) along each cable are also present. The fundamental cabling architecture is also maintained during septal development as part of cell division. We propose a coiled-coil model for peptidoglycan architecture encompassing our data and recent evidence concerning the biosynthetic machinery for this essential polymer. |
| | |
Authors:
|
Emma J Hayhurst; Lekshmi Kailas; Jamie K Hobbs; Simon J Foster |
Related Documents
:
|
17209034 - Porins are required for uptake of phosphates by mycobacterium smegmatis. 4570614 - Temperature-sensitive divisionless mutant of bacillus subtilis defective in the initiat... 7056584 - Relationship between cell wall synthesis in propionibacterium acnes and ability to stim... 8550464 - Cell wall sorting of lipoproteins in staphylococcus aureus. 9345284 - Mtdna mutations confer cellular sensitivity to oxidant stress that is partially rescued... 15767274 - A dual-fluorescence reporter system for high-throughput clone characterization and sele... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-10 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-09-24 Completed Date: 2008-10-22 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 14603-8 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
chemistry*,
cytology*,
genetics Bacterial Proteins / genetics Cell Wall / chemistry* Chromatography, Gel Microscopy, Atomic Force Models, Biological Mutation Peptidoglycan / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
|
GR077544AIA//Wellcome Trust; //Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/MreC protein, Bacteria; 0/Peptidoglycan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pim-1 kinase antagonizes aspects of myocardial hypertrophy and compensation to pathological pressure...
Next Document: Effects of local mRNA structure on posttranscriptional gene silencing.