Document Detail


Cell wall growth during differentiation of Proteus swarmers.
MedLine Citation:
PMID:  4889269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes the process by which the cell wall of Proteus mirabilis, as measured by the presence of the O antigen, develops during the differentiation of swarmers from short cells on an agar surface. The sequence was followed by fluorescent-antibody staining, with both the direct and reverse methods. When the organisms were labeled with fluorescent antibody by the direct method, they showed a progressive diminution of the marker along the cell surface and some increase in the length of the bacteria. However, the label had become completely diluted out before typical swarmers developed. When the bacteria were exposed initially to unlabeled antibody by the reverse technique, and then incubated with fluorescent antibody, they showed a progressive increase both in the intensity of the label along their entire periphery and in cellular length, culminating in the formation of swarmers. It is concluded that in P. mirabilis, as in the few other gram-negative bacteria examined so far, cell wall synthesis takes place diffusely, i.e., by intercalation of new with old components along the length of the cell wall.
Authors:
J F Hoeniger; E A Cinits
Related Documents :
7666189 - Early ontogeny of the secondary proliferative population of the embryonic murine cerebr...
17397129 - Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in ...
2582639 - Pneumatization of the posteromedial air-cell tract.
16524899 - Fission yeast rho5p gtpase is a functional paralogue of rho1p that plays a role in surv...
18000679 - In situ analysis by microspectroscopy reveals triterpenoid compositional patterns withi...
8449879 - Possible role of a choline-containing teichoic acid in the maintenance of normal cell s...
20049859 - Distinct proteomic features of two fibrogenic liver cell populations: hepatic stellate ...
16175609 - Antioxidative function of l-fabp in l-fabp stably transfected chang liver cells.
23565869 - Curcumin loaded nipaam/vp/peg-a nanoparticles: physicochemical and chemopreventive prop...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  98     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1969 Apr 
Date Detail:
Created Date:  1969-06-19     Completed Date:  1969-06-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  226-31     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / metabolism
Antigens / biosynthesis
Cell Differentiation
Cell Wall / growth & development*
Fluorescent Antibody Technique
Methods
Microscopy, Fluorescence
Proteus / cytology*
Rabbits
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conducto...
Next Document:  Analytical studies on regeneration of protoplasts of Geotrichum candidum by quantitative thin-layer-...