Document Detail


Cell wall fraction of Enterococcus hirae ameliorates TNF-alpha-induced barrier impairment in the human epithelial tight junction.
MedLine Citation:
PMID:  18298454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The evaluation of the effects of Enterococcus hirae, an intestinal bacterium in the adjacent mucosa (mucosal bacterium), on tumour necrosis factor-alpha (TNF-alpha)-induced barrier impairment in human epithelial Caco-2 cells. METHODS AND RESULTS: The filter-grown Caco-2 monolayers were used as an intestinal epithelial model system. In Caco-2 cells, heat-killed E. hirae ATCC 9790(T) suppressed the TNF-alpha-induced barrier impairment and increase in interleukin-8 (IL-8) secretion, but lipase- and mutanolysin-treated E. hirae ATCC 9790(T) did not have these effects. It was demonstrated that lipoteichoic acid (LTA) from E. hirae ATCC 9790(T) is responsible for Caco-2 cells' recovery from TNF-alpha-induced impairments. In addition, Caco-2 cells had the same response to Toll-like receptor 2 (TLR2) ligand, Pam(3)Cys-Ser-(Lys)(4) as they did to LTA. Increased expression of zonula occludens-1 was observed by the addition of E. hirae ATCC 9790(T) to TNF-alpha-treated Caco-2 cells, and decreased expression of myosin light chain kinase was observed by the addition of LTA and Pam(3)Cys-Ser-(Lys)(4); this, in turn, led to barrier enforcement. CONCLUSIONS: Enterococcus hirae ATCC 9790(T) cell wall fractions, such as LTA, protect against intestinal impairment by regulation of epithelial tight junction via TLR2 signalling. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus hirae could be useful in the treatment of inflammatory bowel disease, as well as other intestinal disorders.
Authors:
E Miyauchi; H Morita; J Okuda; T Sashihara; M Shimizu; S Tanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-22
Journal Detail:
Title:  Letters in applied microbiology     Volume:  46     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-20     Completed Date:  2008-04-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  469-76     Citation Subset:  IM    
Affiliation:
Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Cell Wall / chemistry,  immunology*
Enterococcus / chemistry,  immunology*
Epithelial Cells / drug effects,  microbiology*
Humans
Interleukin-8 / biosynthesis
Lipopeptides
Lipopolysaccharides / immunology,  isolation & purification
Membrane Proteins / biosynthesis
Myosin-Light-Chain Kinase / biosynthesis
Peptides / immunology
Phosphoproteins / biosynthesis
Teichoic Acids / immunology,  isolation & purification
Tight Junctions / drug effects,  microbiology*
Tumor Necrosis Factor-alpha / immunology*
Chemical
Reg. No./Substance:
0/Interleukin-8; 0/Lipopeptides; 0/Lipopolysaccharides; 0/Membrane Proteins; 0/Pam(3)CSK(4) peptide; 0/Peptides; 0/Phosphoproteins; 0/Teichoic Acids; 0/Tumor Necrosis Factor-alpha; 0/zonula occludens-1 protein; 56411-57-5/lipoteichoic acid; EC 2.7.11.18/Myosin-Light-Chain Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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