| Cell wall fraction of Enterococcus hirae ameliorates TNF-alpha-induced barrier impairment in the human epithelial tight junction. | |
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MedLine Citation:
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PMID: 18298454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: The evaluation of the effects of Enterococcus hirae, an intestinal bacterium in the adjacent mucosa (mucosal bacterium), on tumour necrosis factor-alpha (TNF-alpha)-induced barrier impairment in human epithelial Caco-2 cells. METHODS AND RESULTS: The filter-grown Caco-2 monolayers were used as an intestinal epithelial model system. In Caco-2 cells, heat-killed E. hirae ATCC 9790(T) suppressed the TNF-alpha-induced barrier impairment and increase in interleukin-8 (IL-8) secretion, but lipase- and mutanolysin-treated E. hirae ATCC 9790(T) did not have these effects. It was demonstrated that lipoteichoic acid (LTA) from E. hirae ATCC 9790(T) is responsible for Caco-2 cells' recovery from TNF-alpha-induced impairments. In addition, Caco-2 cells had the same response to Toll-like receptor 2 (TLR2) ligand, Pam(3)Cys-Ser-(Lys)(4) as they did to LTA. Increased expression of zonula occludens-1 was observed by the addition of E. hirae ATCC 9790(T) to TNF-alpha-treated Caco-2 cells, and decreased expression of myosin light chain kinase was observed by the addition of LTA and Pam(3)Cys-Ser-(Lys)(4); this, in turn, led to barrier enforcement. CONCLUSIONS: Enterococcus hirae ATCC 9790(T) cell wall fractions, such as LTA, protect against intestinal impairment by regulation of epithelial tight junction via TLR2 signalling. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus hirae could be useful in the treatment of inflammatory bowel disease, as well as other intestinal disorders. |
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Authors:
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E Miyauchi; H Morita; J Okuda; T Sashihara; M Shimizu; S Tanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-22 |
Journal Detail:
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Title: Letters in applied microbiology Volume: 46 ISSN: 1472-765X ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-20 Completed Date: 2008-04-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 469-76 Citation Subset: IM |
Affiliation:
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Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caco-2 Cells Cell Wall / chemistry, immunology* Enterococcus / chemistry, immunology* Epithelial Cells / drug effects, microbiology* Humans Interleukin-8 / biosynthesis Lipopeptides Lipopolysaccharides / immunology, isolation & purification Membrane Proteins / biosynthesis Myosin-Light-Chain Kinase / biosynthesis Peptides / immunology Phosphoproteins / biosynthesis Teichoic Acids / immunology, isolation & purification Tight Junctions / drug effects, microbiology* Tumor Necrosis Factor-alpha / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-8; 0/Lipopeptides; 0/Lipopolysaccharides; 0/Membrane Proteins; 0/Pam(3)CSK(4) peptide; 0/Peptides; 0/Phosphoproteins; 0/Teichoic Acids; 0/Tumor Necrosis Factor-alpha; 0/zonula occludens-1 protein; 56411-57-5/lipoteichoic acid; EC 2.7.11.18/Myosin-Light-Chain Kinase |
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