Document Detail


Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches.
MedLine Citation:
PMID:  18806214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. Here we present the first study of the cell wall of the oomycete Aphanomyces euteiches, a major parasite of legume plants. Biochemical analyses demonstrated the presence of ca. 10% N-acetyl-D-glucosamine (GlcNAc) in the cell wall. Further characterization of the GlcNAc-containing material revealed that it corresponds to noncrystalline chitosaccharides associated with glucans, rather than to chitin per se. Two putative chitin synthase (CHS) genes were identified by data mining of an A. euteiches expressed sequence tag collection and Southern blot analysis, and full-length cDNA sequences of both genes were obtained. Phylogeny analysis indicated that oomycete CHS diversification occurred before the divergence of the major oomycete lineages. Remarkably, lectin labeling showed that the Aphanomyces euteiches chitosaccharides are exposed at the cell wall surface, and study of the effect of the CHS inhibitor nikkomycin Z demonstrated that they are involved in cell wall function. These data open new perspectives for the development of antioomycete drugs and further studies of the molecular mechanisms involved in the recognition of pathogenic oomycetes by the host plants.
Authors:
Ilham Badreddine; Claude Lafitte; Laurent Heux; Nicholas Skandalis; Zacharoula Spanou; Yves Martinez; Marie-Thérèse Esquerré-Tugayé; Vincent Bulone; Bernard Dumas; Arnaud Bottin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-19
Journal Detail:
Title:  Eukaryotic cell     Volume:  7     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2008-11-26     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1980-93     Citation Subset:  IM    
Affiliation:
UMR 5546, CNRS-Université Paul-Sabatier, 24 Chemin de Borde-Rouge, BP 42617, Auzeville, F-31326 Castanet-Tolosan, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU447431;  EU522489
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Aphanomyces / chemistry,  classification,  genetics,  metabolism*
Cell Wall / genetics,  metabolism*
Chitin Synthase / chemistry,  genetics,  metabolism
Chitosan / metabolism*
Fabaceae / microbiology*
Fungal Proteins / chemistry,  genetics,  metabolism
Molecular Sequence Data
Phylogeny
Plant Diseases / microbiology*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Fungal Proteins; 9012-76-4/Chitosan; EC 2.4.1.16/Chitin Synthase
Comments/Corrections

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