| Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches. | |
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MedLine Citation:
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PMID: 18806214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. Here we present the first study of the cell wall of the oomycete Aphanomyces euteiches, a major parasite of legume plants. Biochemical analyses demonstrated the presence of ca. 10% N-acetyl-D-glucosamine (GlcNAc) in the cell wall. Further characterization of the GlcNAc-containing material revealed that it corresponds to noncrystalline chitosaccharides associated with glucans, rather than to chitin per se. Two putative chitin synthase (CHS) genes were identified by data mining of an A. euteiches expressed sequence tag collection and Southern blot analysis, and full-length cDNA sequences of both genes were obtained. Phylogeny analysis indicated that oomycete CHS diversification occurred before the divergence of the major oomycete lineages. Remarkably, lectin labeling showed that the Aphanomyces euteiches chitosaccharides are exposed at the cell wall surface, and study of the effect of the CHS inhibitor nikkomycin Z demonstrated that they are involved in cell wall function. These data open new perspectives for the development of antioomycete drugs and further studies of the molecular mechanisms involved in the recognition of pathogenic oomycetes by the host plants. |
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Authors:
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Ilham Badreddine; Claude Lafitte; Laurent Heux; Nicholas Skandalis; Zacharoula Spanou; Yves Martinez; Marie-Thérèse Esquerré-Tugayé; Vincent Bulone; Bernard Dumas; Arnaud Bottin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-19 |
Journal Detail:
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Title: Eukaryotic cell Volume: 7 ISSN: 1535-9786 ISO Abbreviation: Eukaryotic Cell Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-05 Completed Date: 2008-11-26 Revised Date: 2013-06-05 |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1980-93 Citation Subset: IM |
Affiliation:
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UMR 5546, CNRS-Université Paul-Sabatier, 24 Chemin de Borde-Rouge, BP 42617, Auzeville, F-31326 Castanet-Tolosan, France. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU447431; EU522489 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Aphanomyces / chemistry, classification, genetics, metabolism* Cell Wall / genetics, metabolism* Chitin Synthase / chemistry, genetics, metabolism Chitosan / metabolism* Fabaceae / microbiology* Fungal Proteins / chemistry, genetics, metabolism Molecular Sequence Data Phylogeny Plant Diseases / microbiology* Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 9012-76-4/Chitosan; EC 2.4.1.16/Chitin Synthase |
| Comments/Corrections | |
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