Document Detail


Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth.
MedLine Citation:
PMID:  22760472     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell wall isolated from pea roots was used to separate and characterize two fractions possessing class III peroxidase activity: (i) ionically bound proteins and (ii) covalently bound proteins. Modified SDS-PAGE separated peroxidase isoforms by their apparent molecular weights: four bands of 56, 46, 44, and 41kDa were found in the ionically bound fraction (iPOD) and one band (70kDa) was resolved after treatment of the cell wall with cellulase and pectinase (cPOD). Isoelectric focusing (IEF) patterns for iPODs and cPODs were significantly different: five iPODs with highly cationic pI (9.5-9.2) were detected, whereas the nine cPODs were anionic with pI values between pH 3.7 and 5. iPODs and cPODs showed rather specific substrate affinity and different sensitivity to inhibitors, heat, and deglycosylation treatments. Peroxidase and oxidase activities and their IEF patterns for both fractions were determined in different zones along the root and in roots of different ages. New iPODs with pI 9.34 and 9.5 were induced with root growth, while the activity of cPODs was more related to the formation of the cell wall in non-elongating tissue. Treatment with auxin that inhibits root growth led to suppression of iPOD and induction of cPOD. A similar effect was obtained with the widely used elicitor, chitosan, which also induced cPODs with pI 5.3 and 5.7, which may be specifically related to pathogen defence. The differences reported here between biochemical properties of cPOD and iPOD and their differential induction during development and under specific treatments implicate that they are involved in specific and different physiological processes.
Authors:
Biljana M Kukavica; Sonja D Veljovicc-Jovanovicc; Ljiljana Menckhoff; Sabine Lüthje
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-03
Journal Detail:
Title:  Journal of experimental botany     Volume:  63     ISSN:  1460-2431     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2013-04-17     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  4631-45     Citation Subset:  IM    
Affiliation:
University of Banja Luka, Faculty of Science and Mathematics Mladena Stojanovicć 2, 78000 Banja Luka Bosnia and Herzegovina.
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MeSH Terms
Descriptor/Qualifier:
Anions / metabolism
Cations / metabolism
Cell Wall / enzymology*
Chelating Agents / pharmacology
Chitosan / pharmacology
Concanavalin A / pharmacology
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors / pharmacology
Glycosylation
Indoleacetic Acids / pharmacology
Isoelectric Focusing
Isoenzymes / antagonists & inhibitors,  chemistry,  isolation & purification,  metabolism
Kinetics
Lectins / pharmacology
Molecular Weight
Oxidation-Reduction
Peas / drug effects,  enzymology*,  growth & development
Peroxidase / antagonists & inhibitors,  chemistry,  isolation & purification,  metabolism*
Plant Growth Regulators / pharmacology
Plant Proteins / antagonists & inhibitors,  chemistry,  isolation & purification,  metabolism
Plant Roots / drug effects,  enzymology*,  growth & development
Chemical
Reg. No./Substance:
0/Anions; 0/Cations; 0/Chelating Agents; 0/Enzyme Inhibitors; 0/Indoleacetic Acids; 0/Isoenzymes; 0/Lectins; 0/Plant Growth Regulators; 0/Plant Proteins; 11028-71-0/Concanavalin A; 9012-76-4/Chitosan; EC 1.11.1.7/Peroxidase
Comments/Corrections

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