Document Detail


Cell volume regulation of rabbit cortical collecting tubule in anisotonic media.
MedLine Citation:
PMID:  2360659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Volume regulation of nonperfused rabbit cortical collecting tubules in anisotonic bathing media was examined in vitro. When media osmolality is abruptly increased by 150 mosmol/kgH2O with the addition of NaCl, tubules shrink by 20% but do not volume regulate. However, volume regulatory increase (VRI) is observed when 1 mM butyrate is present in the bathing media or when tubules are pretreated with hypotonic media. When media osmolality is increased, butyrate-treated tubules shrink to 74% of their isotonic control volume. As evidence of volume regulation, butyrate-treated tubules swell while still bathed in hypertonic media, recovering in 30 min 78% of the volume lost due to osmotic shrinkage. The butyrate effect requires external Na+ and is inhibited by amiloride. When media osmolality is lowered to 150 mosmol/kgH2O, nonbutyrate tubules swell before showing typical volume regulatory decrease. When these tubules are returned to isotonic media, they immediately shrink to 78% of control volume before showing evidence of VRI. These results suggest that, under the appropriate conditions, cortical collecting tubules are capable of VRI.
Authors:
E Natke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-02     Completed Date:  1990-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F1657-65     Citation Subset:  IM    
Affiliation:
Department of Medicine/Nephrology, Winthrop-University Hospital, Mineola, New York 11501.
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MeSH Terms
Descriptor/Qualifier:
Amiloride / pharmacology
Animals
Bicarbonates / pharmacology
Buffers
Butyric Acid
Butyric Acids / antagonists & inhibitors,  pharmacology
Culture Media
Hypotonic Solutions
Isotonic Solutions
Kidney Cortex
Kidney Tubules / cytology*
Kidney Tubules, Collecting / cytology*
Osmosis
Rabbits
Saline Solution, Hypertonic / pharmacology
Sodium / pharmacology
Urea / pharmacology
Chemical
Reg. No./Substance:
0/Bicarbonates; 0/Buffers; 0/Butyric Acids; 0/Culture Media; 0/Hypotonic Solutions; 0/Isotonic Solutions; 0/Saline Solution, Hypertonic; 107-92-6/Butyric Acid; 2609-46-3/Amiloride; 57-13-6/Urea; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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