Document Detail

Cell volume regulation in the proximal convoluted tubule.
MedLine Citation:
PMID:  6812436     Owner:  NLM     Status:  MEDLINE    
To evaluate the effect of hyper- and hypotonicity on proximal convoluted tubule (PCT) cell volume, nonperfused PCT were studied in vitro with hypertonic solutions containing sodium chloride, urea, or mannitol (450 mosmol/kg H2O) and with hypotonic low sodium chloride solutions (160 mosmol/kg H2O). When the tubules were subjected to hypertonic peritubular solutions containing NaCl, cell volume immediately decreased by 15.5% and remained constant throughout the experimental period (60 min). With mannitol, the initial decrease was identical to that with NaCl (17.7%), but the PCT volume increased slightly during the experimental period. With urea, the decrease in cell volume was smaller (7%) and transient. In hypotonicity, the PCT swelled rapidly, but this swelling was followed by a rapid regulatory phase in which PCT volume nearly returned to control values after less than 10 min. With a potassium-free peritubular medium or 10(-3) M ouabain, the regulatory phase of hypotonicity completely disappeared, whereas the cells did not maintain their reduced volume in NaCl-induced hypertonicity. These results suggest that Na-K-ATPase plays an important role in the maintenance of a reduced cellular volume in hypertonicity and in the regulatory phase of hypotonicity, probably by an active extrusion of sodium and water from the cell.
J Gagnon; D Ouimet; H Nguyen; R Laprade; C Le Grimellec; S Carrière; J Cardinal
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  243     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1982-12-03     Completed Date:  1982-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F408-15     Citation Subset:  IM    
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MeSH Terms
Kidney Tubules, Proximal / cytology,  drug effects,  physiology*
Mannitol / pharmacology
Osmolar Concentration
Ouabain / pharmacology
Sodium / pharmacology
Sodium Chloride / pharmacology
Urea / pharmacology
Reg. No./Substance:
57-13-6/Urea; 630-60-4/Ouabain; 69-65-8/Mannitol; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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