Document Detail


Cell volume regulation in Mycoplasma gallisepticum.
MedLine Citation:
PMID:  4030694     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mycoplasma gallisepticum cells incubated in 250 mM NaCl solutions in the absence of glucose showed a progressive fall in intracellular ATP concentration over a period of 2 to 3 h. When the ATP level fell below 40 microM the cell began to swell and become progressively permeable to [14C]inulin and leak intracellular protein and nucleotides. The addition of nondiffusable substances such as MgSO4 or disaccharides prevented swelling, suggesting that NaCl (and water) entry was due to Gibbs-Donnan forces. The addition of glucose after the initiation of cell swelling increased intracellular ATP, induced cell shrinkage, and prevented the release of intracellular components. The ATPase inhibitor dicyclohexylcarbodiimide, which collapsed the chemical and electrical components of the proton motive force, caused rapid cell swelling in the presence of glucose (and high intracellular ATP levels). Extracellular impermeable solutes such as MgSO4 and disaccharides prevented swelling of dicyclohexylcarbodiimide-treated cells incubated in NaCl. It was postulated that Na+ that diffused into the cell was extruded by an electrogenic Na+-H+ exchange (antiport) energized by the proton motive force established by the dicyclohexylcarbodiimide-sensitive H+-ATPase.
Authors:
C Linker; T H Wilson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  163     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-10-23     Completed Date:  1985-10-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1243-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Body Fluids / analysis*
Dicyclohexylcarbodiimide / pharmacology
Glucose / pharmacology
Intracellular Fluid / analysis*
Kinetics
Mycoplasma / drug effects,  physiology*
Osmolar Concentration
Potassium Chloride / pharmacology
Sodium Chloride / pharmacology
Sucrose / pharmacology
Grant Support
ID/Acronym/Agency:
AM-05736/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
50-99-7/Glucose; 538-75-0/Dicyclohexylcarbodiimide; 56-65-5/Adenosine Triphosphate; 57-50-1/Sucrose; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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