Document Detail


Cell volume and dry weight of cultured Tetrahymena.
MedLine Citation:
PMID:  2777928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultures of Tetrahymena pyriformis, T. hegewishi and T. malaccensis have been studied with regard to control of cell volume and cellular dry weight. Cell volume was measured on cells suspended in 0.9% sodium chloride + 0.1% sodium azide using a Multisizer cell counter (Coulter). Tetrahymena were grown at different temperatures and under various up- or downshift conditions. In all cases the changes in cell volume are paralleled by changes in cellular dry weight. The volume and the dry weight of a Tetrahymena cell are determined by the particular medium and the growth temperature. Large cells are seen in concentrated media and at low growth temperatures resulting in cell volumes up to 17,000 microns 3, whereas starving cells decrease gradually towards 700 microns 3 or even smaller sizes. It is proposed that lag phase observed at up- and downshift is to a large extent due to the necessary adjustment of the cell volume to the new conditions.
Authors:
P Hellung-Larsen; A P Andersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  92 ( Pt 2)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Affiliation:
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cells, Cultured
Potassium / analysis
Sodium / analysis
Tetrahymena / analysis,  cytology*
Chemical
Reg. No./Substance:
7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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