Document Detail

Cell viability effects of triamcinolone acetonide and preservative vehicle formulations.
MedLine Citation:
PMID:  16424540     Owner:  NLM     Status:  MEDLINE    
AIM: To assess the effect of triamcinolone acetonide and preservative vehicle formulations on human retinal pigment epithelium (ARPE19) cells over a range of concentrations.
METHODS: Triamcinolone acetonide, in its trade and preservative free formulations, along with the preservative vehicle were added to ARPE19 cell cultures in various concentrations (0.01-1.0 mg/ml). Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at day 5 after exposure. Functionality of the cultured ARPE19 cell line was confirmed by exposure to a previously characterised toxic agent, tamoxifen.
RESULTS: The ARPE19 cell line behaved as predicted with exposure to tamoxifen. All formulations caused significant reductions in ARPE19 cell viability at the highest concentrations (1.0 mg/ml for triamcinolone preparations and undiluted vehicle). Cell viability was reduced to the greatest degree in trade formulation triamcinolone, less so by the vehicle, and least by preservative free triamcinolone. At lower concentrations no significant effect on cell viability was observed, although cell viability was found to be inversely proportional to increasing concentration of all tested reagents
CONCLUSIONS: Both the trade and preservative free formulations of triamcinolone acetonide as well as the vehicle result in cell loss at in vitro concentrations of 1 mg/ml. Although this represents theoretical vitreous concentrations achieved with current widespread therapeutic use it probably does not indicate the actual exposure of cells in their biological milieu. That cell viability was reduced most in the trade formulation suggests a possible potentiated inhibitory toxic effect of triamcinolone acetonide and vehicle at higher concentrations.
S Shaikh; S Ho; L A Engelmann; S W Klemann
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  90     ISSN:  0007-1161     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-20     Completed Date:  2006-02-23     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  233-6     Citation Subset:  IM    
44 Lake Beauty Drive Suite 300, Orlando, FL 32806, USA.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology*
Antineoplastic Agents, Hormonal / pharmacology
Cell Line
Cell Survival / drug effects
Culture Media
Microscopy, Phase-Contrast / methods
Pharmaceutical Vehicles
Pigment Epithelium of Eye / cytology,  drug effects*
Preservatives, Pharmaceutical / pharmacology*
Retina / cytology,  drug effects
Tamoxifen / pharmacology
Triamcinolone Acetonide / pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Antineoplastic Agents, Hormonal; 0/Culture Media; 0/Pharmaceutical Vehicles; 0/Preservatives, Pharmaceutical; 10540-29-1/Tamoxifen; 76-25-5/Triamcinolone Acetonide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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