Document Detail

Cell viability and angiogenic potential of a bioartificial adipose substitute.
MedLine Citation:
PMID:  23166045     Owner:  NLM     Status:  Publisher    
An implantable scaffold pre-seeded with cells needs to remain viable and encourage rapid angiogenesis in order to replace injured tissues, especially for tissue defect repairs. We created a bioartificial adipose graft composed of an electrospun 3D nanofibrous scaffold and fat tissue excised from New Zealand white rabbits. Cell viability and angiogenesis potential of the bioartificial substitute were examined during four weeks of culture in Dulbecco's Modified Eagle Medium by immunohistochemical staining with LIVE/DEAD® cell kit and PECAM-1 antibody, respectively. In addition, a Matrigel® assay was performed to examine the possibility of blood vessels sprouting from the bioartificial graft. Our results showed that cells within the graft were viable and vascular tubes were present at week 4, while cells in a fat tissue block were dead in vitro. In addition, capillaries were observed sprouting from the graft into the Matrigel, demonstrating its angiogenic potential. We expect that improved cell viability and angiogenesis in the bioartificial substitute, compared to intact autologous graft, could potentially contribute to its survival following implantation. Copyright © 2012 John Wiley & Sons, Ltd.
Anitha Panneerselvan; Luong Th Nguyen; Yan Su; Wee Eong Teo; Susan Liao; Seeram Ramakrishna; Ching Wan Chan
Related Documents :
17604135 - Erbeta shifts from mitochondria to nucleus during estrogen-induced neoplastic transform...
19493305 - The alternative oxidase, a tool for compensating cytochrome c oxidase deficiency in hum...
11115375 - Electrical coupling and plasticity of the mitochondrial network.
6825225 - Metabolic cost of the stimulated beating of isolated adult rat heart cells in suspension.
10491395 - Listeria monocytogenes exploits normal host cell processes to spread from cell to cell.
8666165 - Neurohormone melatonin prevents cell damage: effect on gene expression for antioxidant ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-20
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  -     ISSN:  1932-7005     ISO Abbreviation:  J Tissue Eng Regen Med     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Department of General Surgery, National University Healthcare System, Singapore.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Inhibiting periapical lesions through AAV-RNAi silencing of cathepsin K.
Next Document:  External Regulation of Controlled Polymerizations.