Document Detail


Cell uptake and localization studies of squaramide based fluorescent probes.
MedLine Citation:
PMID:  25036647     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell internalization is a major issue in drug design. Although squaramide-based compounds are driving much attention because their interesting bioactivities, cell uptake and trafficking within the cell of this type of compounds are still unknown. In order to monitor the cell internalization process of cycloscuaramide compounds we have prepared two fluorescent probes by covalently linking a fluorescent dye (BODIPY derivative or fluorescein) to a non-cytotoxic cyclosquaramide framework. These two probes (C2-BDP and C2-FITC) rapidly internalize across live cell membranes through endocytic receptor-mediated mechanisms. Due to its higher fluorescence and photochemical stability, C2-BDP is a superior dye than C2-FITC. C2-BDP remains sequestered in late endosomes allowing their fast and selective imaging in various live cell types. Cyclosquaramide-cell membrane interactions facilitate cell uptake and have been investigated by binding studies in solution as well as in live cells. Cyclosquaramide 1 (C2-BDP) can be used as a highly fluorescent probe for the rapid and selective imaging of late endosomes in live cells.
Authors:
Carmen Rotger; Angel Sampedro; Ruth Villalonga; Manuel Vega; Guillem Ramis; Silvia Fernández de Mattos; Priam Villalonga; Antoni Costa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-18
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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