Document Detail

Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer.
MedLine Citation:
PMID:  17234370     Owner:  NLM     Status:  MEDLINE    
Macromolecular crowding and the presence of organelles in the cytosol present barriers to particle mobility, such that it is unclear how nano-carriers can deliver their active agents to the nucleus. In this work a sixth generation amino terminated polyamide polylysine dendrimer (Gly-Lys(63) (NH(2))(64)) (MW 8149, diameter 6.5 nm) which is fluorescent allowed the study of nuclear uptake and mobility in living lung carcinoma (SK/MES-1) and colon adenocarcinoma (Caco-2) cells. The dendrimer is found within 25-45 min of incubation inside the cell nuclei. Living cells were then used to develop a method for the dynamic nuclear uptake study using confocal microscopy. The dynamic uptake of the dendrimer demonstrated here allowed the apparent cytoplasmic diffusion coefficient (D) of the dendrimer to be calculated. Values were found in the range 5.99 x 10(-11)cm(2)s(-1) (SK/MES-1 cells) to 9.82 x 10(-11)cm(2)s(-1) (Caco-2 cells). The difference must reflect variation in the intracellular architecture of the cell types.
Pakatip Ruenraroengsak; Khuloud T Al-Jamal; Nicholas Hartell; Kevin Braeckmans; Stefaan C De Smedt; Alexander T Florence
Publication Detail:
Type:  Journal Article     Date:  2006-12-14
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  331     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-04-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  215-9     Citation Subset:  IM    
Centre for Drug Delivery Research, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1 AX, UK.
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MeSH Terms
Biological Transport
Cell Line, Tumor
Cell Membrane Permeability
Cytoplasm / metabolism
Dendrimers / chemistry,  pharmacokinetics*
Drug Carriers
Microscopy, Confocal
Nanoparticles / therapeutic use*
Particle Size
Reg. No./Substance:
0/Dendrimers; 0/Drug Carriers; 0/Nylons; 25104-18-1/Polylysine

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