Document Detail


Cell type-specific protein and transcription profiles implicate periarbuscular membrane synthesis as an important carbon sink in the mycorrhizal symbiosis.
MedLine Citation:
PMID:  22499167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of an arbuscular mycorrhizal (AM) symbiosis is a non-synchronous process with typical mycorrhizal root containing different symbiotic stages at one time. Methods providing cell type-specific resolution are therefore required to separate these stages and analyze each particular structure independently from each other. We established an experimental system for analyzing specific proteomic changes in arbuscule-containing cells of Glomus intraradices colonized Medicago truncatula roots. The combination of laser capture microdissection (LCM) and liquid chromatography-tandem mass chromatography (LC-MS/MS) allowed the identification of proteins with specific or increased expression in arbuscule-containing cells. Consistent with previous transcriptome data, the proteome of arbuscule-containing cells showed an increased number of proteins involved in lipid metabolism, most likely related to the synthesis of the periarbuscular membrane. In addition, transcriptome data of non-colonized cells of mycorrhizal roots suggest mobilization of carbon resources and their symplastic transport toward arbuscule-containing cells for the synthesis of periarbuscular membranes. This highlights the periarbuscular membrane as important carbon sink in the mycorrhizal symbiosis.
Authors:
Nicole Gaude; Waltraud X Schulze; Philipp Franken; Franziska Krajinski
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Publication Detail:
Type:  Journal Article     Date:  2012-04-01
Journal Detail:
Title:  Plant signaling & behavior     Volume:  7     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-09-17     Completed Date:  2013-02-07     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  461-4     Citation Subset:  IM    
Affiliation:
Max Planck Institute of Molecular Plant Physiology, Golm, Germany.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport / genetics
Carbohydrate Metabolism / genetics
Carbon Sequestration / genetics*
Gene Expression Profiling*
Gene Expression Regulation, Plant
Glomeromycota / physiology
Laser Capture Microdissection
Lipid Metabolism / genetics
Medicago truncatula / cytology,  genetics,  microbiology*
Mycorrhizae / physiology*
Plant Proteins / genetics,  metabolism*
Plant Roots / cytology*,  genetics,  microbiology
Proteome / metabolism
RNA, Messenger / genetics,  metabolism
Symbiosis / genetics*
Chemical
Reg. No./Substance:
0/Plant Proteins; 0/Proteome; 0/RNA, Messenger
Comments/Corrections

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