Document Detail

Cell-type-specific function of the C-type natriuretic peptide gene promoter in rat anterior pituitary-derived cultured cell lines.
MedLine Citation:
PMID:  8321215     Owner:  NLM     Status:  MEDLINE    
The promoter function of the human C-type natriuretic peptide (CNP) gene in various cultured cells was examined by transient transfection assays. The CNP promoter functioned very effectively in GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, but functioned much less effectively in GH1 cells, another type of rat pituitary-derived cell with a somatotroph phenotype, and rat primary cardiocytes. The CNP promoter did not function at all in other cells, including AtT20 cells of murine pituitary corticotroph origin. Functional analyses of the deleted promoters with various 5' deletion breakpoints revealed the existence of at least two negative and one positive regulatory regions. Within the positive regulatory region (positions -54 to -19), which conferred 90% of the promoter activity in GH3 cells, two equipotent GC-rich cis elements (positions -49 to -45 and -40 to -35) were identified. Both sites shared half of the promoter activity and binding properties to the nuclear protein in GH3 cells. Rat anterior pituitary tissue contained the binding protein of the identified cis element, which was identical or similar to that of GH3 cells. With Southwestern (DNA-protein) analysis, a 70-kDa specific binding protein distinct from known factors such as SP-1, AP-2, and Pit-1 was identified in the nuclear extract of GH3 cells.
S Ohta; Y Shimekake; K Nagata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  13     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-07-30     Completed Date:  1993-07-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4077-86     Citation Subset:  IM    
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka 553, Japan.
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MeSH Terms
Atrial Natriuretic Factor / genetics*,  metabolism
Base Sequence
Blotting, Southern
Cell Line
Cells, Cultured
Chloramphenicol O-Acetyltransferase / genetics,  metabolism
Cloning, Molecular
Molecular Sequence Data
Natriuretic Peptide, C-Type
Nerve Tissue Proteins / genetics*,  metabolism
Organ Specificity / genetics
Pituitary Gland, Anterior / cytology,  metabolism*
Promoter Regions, Genetic*
Restriction Mapping
Transcription, Genetic
Reg. No./Substance:
0/Nerve Tissue Proteins; 127869-51-6/Natriuretic Peptide, C-Type; 85637-73-6/Atrial Natriuretic Factor; 9007-49-2/DNA; EC O-Acetyltransferase

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