| Cell-type-specific function of the C-type natriuretic peptide gene promoter in rat anterior pituitary-derived cultured cell lines. | |
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MedLine Citation:
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PMID: 8321215 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The promoter function of the human C-type natriuretic peptide (CNP) gene in various cultured cells was examined by transient transfection assays. The CNP promoter functioned very effectively in GH3 cells, which originated from the growth hormone-producing tumor of the rat anterior pituitary and somatomammotroph phenotype, but functioned much less effectively in GH1 cells, another type of rat pituitary-derived cell with a somatotroph phenotype, and rat primary cardiocytes. The CNP promoter did not function at all in other cells, including AtT20 cells of murine pituitary corticotroph origin. Functional analyses of the deleted promoters with various 5' deletion breakpoints revealed the existence of at least two negative and one positive regulatory regions. Within the positive regulatory region (positions -54 to -19), which conferred 90% of the promoter activity in GH3 cells, two equipotent GC-rich cis elements (positions -49 to -45 and -40 to -35) were identified. Both sites shared half of the promoter activity and binding properties to the nuclear protein in GH3 cells. Rat anterior pituitary tissue contained the binding protein of the identified cis element, which was identical or similar to that of GH3 cells. With Southwestern (DNA-protein) analysis, a 70-kDa specific binding protein distinct from known factors such as SP-1, AP-2, and Pit-1 was identified in the nuclear extract of GH3 cells. |
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Authors:
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S Ohta; Y Shimekake; K Nagata |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biology Volume: 13 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-07-30 Completed Date: 1993-07-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4077-86 Citation Subset: IM |
Affiliation:
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Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka 553, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrial Natriuretic Factor / genetics*, metabolism Base Sequence Blotting, Southern Cell Line Cells, Cultured Chloramphenicol O-Acetyltransferase / genetics, metabolism Cloning, Molecular DNA Humans Mice Molecular Sequence Data Mutagenesis Natriuretic Peptide, C-Type Nerve Tissue Proteins / genetics*, metabolism Organ Specificity / genetics Pituitary Gland, Anterior / cytology, metabolism* Promoter Regions, Genetic* Rats Restriction Mapping Transcription, Genetic Transfection |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 127869-51-6/Natriuretic Peptide, C-Type; 85637-73-6/Atrial Natriuretic Factor; 9007-49-2/DNA; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase |
| Comments/Corrections | |
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