Document Detail


Cell type-specific expression of beta-carotene 9',10'-monooxygenase in human tissues.
MedLine Citation:
PMID:  15983114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The symmetrically cleaving beta-carotene 15,15'-monooxygenase (BCO1) catalyzes the first step in the conversion of provitamin A carotenoids to vitamin A in the mucosa of the small intestine. This enzyme is also expressed in epithelia in a variety of extraintestinal tissues. The newly discovered beta-carotene 9',10'-monooxygenase (BCO2) catalyzes asymmetric cleavage of carotenoids. To gain some insight into the physiological role of BCO2, we determined the expression pattern of BCO2 mRNA and protein in human tissues. By immunohistochemical analysis it was revealed that BCO2 was detected in cell types that are known to express BCO1, such as epithelial cells in the mucosa of small intestine and stomach, parenchymal cells in liver, Leydig and Sertoli cells in testis, kidney tubules, adrenal gland, exocrine pancreas, and retinal pigment epithelium and ciliary body pigment epithelia in the eye. BCO2 was uniquely detected in cardiac and skeletal muscle cells, prostate and endometrial connective tissue, and endocrine pancreas. The finding that the BCO2 enzyme was expressed in some tissues and cell types that are not sensitive to vitamin A deficiency and where no BCO1 has been detected suggests that BCO2 may also be involved in biological processes other than vitamin A synthesis.
Authors:
Annika Lindqvist; Yu-Guang He; Stefan Andersson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2005-06-27
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  53     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-01     Completed Date:  2005-12-30     Revised Date:  2010-06-02    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1403-12     Citation Subset:  IM    
Affiliation:
University of Texas Southwestern Medical Center, Department of Obstetrics-Gynecology, F2.106 5323 Harry Hines Blvd., Dallas, TX 75390-9032, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Fatty Acid Desaturases / biosynthesis*
Humans
Immunohistochemistry
Organ Specificity
RNA, Messenger / biosynthesis
beta-Carotene 15,15'-Monooxygenase / biosynthesis*,  genetics
Grant Support
ID/Acronym/Agency:
DK-62192/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/RNA, Messenger; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/BCO2 protein, human; EC 1.14.99.36/beta-Carotene 15,15'-Monooxygenase

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