Document Detail


Cell type-specific acquired protection from crystal adherence by renal tubule cells in culture.
MedLine Citation:
PMID:  10201007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Adherence of crystals to the surface of renal tubule epithelial cells is considered an important step in the development of nephrolithiasis. Previously, we demonstrated that functional monolayers formed by the renal tubule cell line, Madin-Darby canine kidney (MDCK), acquire protection against the adherence of calcium oxalate monohydrate crystals. We now examined whether this property is cell type specific. The susceptibility of the cells to crystal binding was further studied under different culture conditions. METHODS: Cell-type specificity and the influence of the growth substrate was tested by comparing calcium oxalate monohydrate crystal binding to LLC-PK1 cells and to two MDCK strains cultured on either permeable or impermeable supports. These cell lines are representative for the renal proximal tubule (LLC-PK1) and distal tubule/collecting duct (MDCK) segments of the nephron, in which crystals are expected to be absent and present, respectively. RESULTS: Whereas relatively large amounts of crystals adhered to subconfluent MDCK cultures, the level of crystal binding to confluent monolayers was reduced for both MDCK strains. On permeable supports, MDCK cells not only obtained a higher level of morphological differentiation, but also acquired a higher degree of protection than on impermeable surfaces. Crystals avidly adhered to LLC-PK1 cells, irrespective of their developmental stage or growth substrate used. CONCLUSIONS: These results show that the prevention of crystal binding is cell type specific and expressed only by differentiated MDCK cells. The anti-adherence properties acquired by MDCK cells may mirror a specific functional characteristic of its in situ equivalent, the renal distal tubule/collecting ducts.
Authors:
C F Verkoelen; B G van der Boom; D J Kok; A B Houtsmuller; P Visser; F H Schröder; J C Romijn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  55     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-07-14     Completed Date:  1999-07-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1426-33     Citation Subset:  IM    
Affiliation:
Department of Urology, Erasmus University, Rotterdam, The Netherlands. Verkoelen@uro.fgg.eur.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Oxalate / metabolism
Cell Adhesion / physiology
Cell Line
Cell Size / physiology
Crystallization
Diffusion Chambers, Culture
Dogs
Kidney Tubules, Collecting / cytology,  metabolism*,  ultrastructure
Kidney Tubules, Proximal / cytology,  metabolism*,  ultrastructure
LLC-PK1 Cells
Microscopy, Confocal
Microscopy, Electron, Scanning
Ouabain / metabolism
Sodium-Potassium-Exchanging ATPase / metabolism
Swine
Time Factors
Chemical
Reg. No./Substance:
25454-23-3/Calcium Oxalate; 630-60-4/Ouabain; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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