Document Detail

Cell type-autonomous and non-autonomous requirements for Dmrt1 in postnatal testis differentiation.
MedLine Citation:
PMID:  17540358     Owner:  NLM     Status:  MEDLINE    
Genes containing the DM domain, a conserved DNA binding motif first found in Doublesex of Drosophila and mab-3 of Caenorhabditis elegans, regulate sexual differentiation in multiple phyla. The DM domain gene Dmrt1 is essential for testicular differentiation in vertebrates. In the mouse, Dmrt1 is expressed in pre-meiotic germ cells and in Sertoli cells, which provide essential support for spermatogenesis. Dmrt1 null mutant mice have severely dysgenic testes in which Sertoli cells and germ cells both fail to differentiate properly after birth. Here we use conditional gene targeting to identify the functions of Dmrt1 in each cell type. We find that Dmrt1 is required in Sertoli cells for their postnatal differentiation, and for germ line maintenance and for meiotic progression. Dmrt1 is required in germ cells for their radial migration to the periphery of the seminiferous tubule where the spermatogenic niche will form, for mitotic reactivation and for survival beyond the first postnatal week. Thus Dmrt1 activity is required autonomously in the Sertoli and germ cell lineages, and Dmrt1 activity in Sertoli cells is also required non-autonomously to maintain the germ line. These results demonstrate that Dmrt1 plays multiple roles in controlling the remodeling and differentiation of the juvenile testis.
Shinseog Kim; Vivian J Bardwell; David Zarkower
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-05-03
Journal Detail:
Title:  Developmental biology     Volume:  307     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-06     Completed Date:  2007-09-05     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  314-27     Citation Subset:  IM    
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.
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MeSH Terms
Base Sequence
Cell Differentiation
DNA Primers / genetics
Mice, Inbred C57BL
Mice, Knockout
Receptors, Androgen / metabolism
Sertoli Cells / cytology,  metabolism
Testis / cytology,  growth & development*,  metabolism
Transcription Factors / deficiency,  genetics,  physiology*
Grant Support
GM59152/GM/NIGMS NIH HHS; R01 GM059152/GM/NIGMS NIH HHS; R01 GM059152-05/GM/NIGMS NIH HHS; R01 GM059152-05S1/GM/NIGMS NIH HHS; R01 GM059152-06/GM/NIGMS NIH HHS; R01 GM059152-07/GM/NIGMS NIH HHS; R01 GM059152-08/GM/NIGMS NIH HHS
Reg. No./Substance:
0/DMRT1 protein; 0/DNA Primers; 0/Receptors, Androgen; 0/Transcription Factors

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