| Cell type-autonomous and non-autonomous requirements for Dmrt1 in postnatal testis differentiation. | |
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MedLine Citation:
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PMID: 17540358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genes containing the DM domain, a conserved DNA binding motif first found in Doublesex of Drosophila and mab-3 of Caenorhabditis elegans, regulate sexual differentiation in multiple phyla. The DM domain gene Dmrt1 is essential for testicular differentiation in vertebrates. In the mouse, Dmrt1 is expressed in pre-meiotic germ cells and in Sertoli cells, which provide essential support for spermatogenesis. Dmrt1 null mutant mice have severely dysgenic testes in which Sertoli cells and germ cells both fail to differentiate properly after birth. Here we use conditional gene targeting to identify the functions of Dmrt1 in each cell type. We find that Dmrt1 is required in Sertoli cells for their postnatal differentiation, and for germ line maintenance and for meiotic progression. Dmrt1 is required in germ cells for their radial migration to the periphery of the seminiferous tubule where the spermatogenic niche will form, for mitotic reactivation and for survival beyond the first postnatal week. Thus Dmrt1 activity is required autonomously in the Sertoli and germ cell lineages, and Dmrt1 activity in Sertoli cells is also required non-autonomously to maintain the germ line. These results demonstrate that Dmrt1 plays multiple roles in controlling the remodeling and differentiation of the juvenile testis. |
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Authors:
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Shinseog Kim; Vivian J Bardwell; David Zarkower |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-05-03 |
Journal Detail:
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Title: Developmental biology Volume: 307 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-06 Completed Date: 2007-09-05 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 314-27 Citation Subset: IM |
Affiliation:
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Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Differentiation DNA Primers / genetics Male Meiosis Mice Mice, Inbred C57BL Mice, Knockout Mitosis Receptors, Androgen / metabolism Sertoli Cells / cytology, metabolism Spermatogenesis Testis / cytology, growth & development*, metabolism Transcription Factors / deficiency, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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GM59152/GM/NIGMS NIH HHS; R01 GM059152/GM/NIGMS NIH HHS; R01 GM059152-05/GM/NIGMS NIH HHS; R01 GM059152-05S1/GM/NIGMS NIH HHS; R01 GM059152-06/GM/NIGMS NIH HHS; R01 GM059152-07/GM/NIGMS NIH HHS; R01 GM059152-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DMRT1 protein; 0/DNA Primers; 0/Receptors, Androgen; 0/Transcription Factors |
| Comments/Corrections | |
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