Document Detail

Cell trafficking of carbon nanotubes based on fluorescence detection.
MedLine Citation:
PMID:  20422387     Owner:  NLM     Status:  MEDLINE    
Cell trafficking of carbon nanotubes (CNTs) is an area of scientific inquiry that has great implications in medicine, biosensing, and environmental science and engineering. The essence of this inquiry resides in the interaction of carbon nanostructures and cell membranes, regulated by the laws of molecular cell biology and the physiochemical properties of the nanostructures. Of equal importance to this inquiry is a description of cellular responses to the integration of man-made materials; yet, how cellular responses may invoke whole-organism level reaction remains unclear. In this chapter, we show three experimental studies, which may be beneficial to obtaining such an understanding. Among the reservoir of methodologies, which have proved of merit, we focus our attention on fluorescence microscopy, one of the most powerful and yet least invasive means of probing nanoparticles in biological systems. Especially, we present the method of fluorescence energy transfer induced between a lysophospholipid molecule and a single-walled CNT upon cellular uptake, and describe coating nanotubes with RNA and suspending fullerenes with phenolic acids for facilitating their translocation across cell membranes and shuttling between cell organelles. Finally, we comment on the perspective of using molecular simulations for facilitating and guiding such experiments.
Monica H Lamm; Pu Chun Ke
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  625     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-27     Completed Date:  2010-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-51     Citation Subset:  IM    
Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, USA.
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MeSH Terms
Cell Membrane / drug effects
Coated Materials, Biocompatible / chemistry*,  pharmacokinetics*,  toxicity
Fluorescence Resonance Energy Transfer
Microscopy, Fluorescence
Nanotubes, Carbon / chemistry*,  toxicity
Sensitivity and Specificity
Tissue Distribution
Tumor Cells, Cultured
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Nanotubes, Carbon

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