Document Detail

Cell tracking using iron oxide fails to distinguish dead from living transplanted cells in the infarcted heart.
MedLine Citation:
PMID:  20187188     Owner:  NLM     Status:  MEDLINE    
Recently, debate has arisen about the usefulness of cell tracking using iron oxide-labeled cells. Two important issues in determining the usefulness of cell tracking with MRI are generally overlooked; first, the effect of graft rejection in immunocompetent models, and second, the necessity for careful histological confirmation of the fate of the labeled cells in the presence of iron oxide. Therefore, both iron oxide-labeled living as well as dead epicardium-derived cells (EPDCs) were investigated in ischemic myocardium of immunodeficient non-obese diabetic (NOD)/acid: non-obese diabetic severe combined immunodeficient (NOD/scid) mice with 9.4T MRI until 6 weeks after surgery, at which time immunohistochemical analysis was performed. In both groups, voids on MRI scans were observed that did not change in number, size, or localization over time. Based on MRI, no distinction could be made between living and dead injected cells. Prussian blue staining confirmed that the hypointense spots on MRI corresponded to iron-loaded cells. However, in the dead-EPDC recipients, all iron-positive cells appeared to be macrophages, while the living-EPDC recipients also contained engrafted iron-loaded EPDCs. Iron labeling is inadequate for determining the fate of transplanted cells in the immunodeficient host, since dead cells produce an MRI signal indistinguishable from incorporated living cells.
E M Winter; B Hogers; L M van der Graaf; A C Gittenberger-de Groot; R E Poelmann; L van der Weerd
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  63     ISSN:  1522-2594     ISO Abbreviation:  Magn Reson Med     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-06-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  817-21     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
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MeSH Terms
Cell Survival
Cells, Cultured
Contrast Media
Ferric Compounds / diagnostic use*
Image Enhancement / methods*
Magnetic Resonance Imaging / methods*
Myocardial Infarction / pathology*,  surgery*
Myocytes, Cardiac / pathology*,  transplantation*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Contrast Media; 0/Ferric Compounds; 1309-37-1/ferric oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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