Document Detail


Cell-to-cell spread of HIV-1 occurs within minutes and may not involve the participation of virus particles.
MedLine Citation:
PMID:  1370739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although virus infections have been classically studied with "cell-free" virion preparations, many animal viruses are able to spread both in vitro and in vivo by inducing cell-cell fusion. An efficient system to monitor the cell-to-cell spread of HIV-1 has been developed employing chronically infected H9 donor cells. Under appropriate conditions of cocultivation with uninfected cells, the synthesis of unintegrated viral DNA, monitored by Southern blot hybridization, occurred between 2 and 4 hr following infection; viral proteins were detected 8 to 12 hr following cocultivation and progeny virions were released into the medium by 16 hr. The use of metabolic inhibitors or specific envelope/receptor antibodies revealed that the cell-to-cell spread of HIV required: (1) gp120-CD4 interaction and (2) reverse transcription. Light and electron microscopy, fluorescent dye redistribution, and soluble CD4 competition experiments all demonstrated that the HIV-induced cell-cell fusion began within 10 to 30 min of cocultivation. Surprisingly, the electron microscopic analyses also suggested that budding or mature virus particles did not participate in this process. Thus the virus-induced cell-cell fusion observed is very likely the result of gp120/gp41 proteins, on the surface of infected cells, interacting with CD4 molecules on uninfected cells. These findings are of immediate importance in understanding the mechanism(s) of HIV-1 transmission in vivo and for the design of effective vaccines and antiviral agents.
Authors:
H Sato; J Orenstein; D Dimitrov; M Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  186     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-26     Completed Date:  1992-02-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  712-24     Citation Subset:  IM; X    
Affiliation:
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD4 / metabolism
Blotting, Western
Cell Fusion / drug effects
Cell Line
DNA, Viral / biosynthesis
DNA-Directed DNA Polymerase / metabolism
Dextran Sulfate / pharmacology
Fluorescent Dyes
HIV Envelope Protein gp120 / metabolism
HIV Reverse Transcriptase
HIV-1 / physiology*
Heparin / pharmacology
Humans
Kinetics
Microscopy, Electron
Models, Biological
RNA-Directed DNA Polymerase / metabolism
T-Lymphocytes / microbiology*,  ultrastructure
Virus Replication
Chemical
Reg. No./Substance:
0/Antigens, CD4; 0/DNA, Viral; 0/Fluorescent Dyes; 0/HIV Envelope Protein gp120; 9005-49-6/Heparin; 9042-14-2/Dextran Sulfate; EC 2.7.7.49/HIV Reverse Transcriptase; EC 2.7.7.49/RNA-Directed DNA Polymerase; EC 2.7.7.7/DNA-Directed DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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