Document Detail

Cell-to-cell movement of turnip crinkle virus is controlled by two small open reading frames that function in trans.
MedLine Citation:
PMID:  9601509     Owner:  NLM     Status:  MEDLINE    
Previous studies on turnip crinkle virus (TCV) have suggested that the two small, centrally located ORFs, conserved in all Carmoviruses, are both required for cell-to-cell movement (Hacker et al., 1992). We now demonstrate that the cell-to-cell movement of TCV is mediated by in trans complementation of the two proteins. First, both of the putative movement proteins (MPs p8 and p9) were shown to be translated in vitro from transcripts representing the 1.7-kb subgenomic RNA. Western blot analysis, using antisera prepared against GST fusion proteins of both genes, was then used to show that the p8 but not the p9 protein accumulated to detectable levels in particulate fractions of infected cells. Cell-to-cell movement of various MP mutants in Arabidopsis was evaluated by in situ hybridization of inoculated leaves. Changes in either of the two MP genes resulted in failure of the mutants to move cell-to-cell. Coat protein was found to be unnecessary for cell-to-cell movement. Complementation of cell-to-cell movement by co-inoculating p8-defective mutants with a p9-defective mutant resulted in delayed systemic infection. In contrast, efficient cell-to-cell movement was achieved when the MP mutants were inoculated into transgenic plants expressing the corresponding functional gene(s). These experiments provide further evidence that both MP genes encoded by Carmoviruses must function in trans in the same cell in order to mediate cell-to-cell movement.
W Z Li; F Qu; T J Morris
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Virology     Volume:  244     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-16     Citation Subset:  IM    
School of Biological Sciences, University of Nebraska-Lincoln 68588-0118, USA.
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MeSH Terms
Arabidopsis / genetics,  virology
Base Sequence
Carmovirus / genetics*,  pathogenicity*,  physiology
DNA Primers / genetics
Gene Expression
Genes, Viral*
Genetic Complementation Test
Genome, Viral
Open Reading Frames
Plant Diseases / virology
Plant Viral Movement Proteins
Plants, Genetically Modified
RNA, Viral / genetics
Vegetables / virology
Viral Proteins / genetics,  physiology
Virulence / genetics,  physiology
Reg. No./Substance:
0/DNA Primers; 0/Plant Viral Movement Proteins; 0/RNA, Viral; 0/Viral Proteins

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