Document Detail


Cell-to-cell fusion, but not virus entry in macrophages by T-cell line tropic HIV-1 strains: a V3 loop-determined restriction.
MedLine Citation:
PMID:  7778305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T-cell lines chronically infected with laboratory adapted, T-cell tropic HIV-1 strains (RF, LAI, CBL-4, and NY5) induced syncytia after cocultivation with primary macrophages. Such fusion-competent strains, however, replicated inefficiently after cell-free infection of macrophage cultures. Evidence based on proviral DNA synthesis, pseudotype penetration, and a V3 loop mutation revealed that cell-free infection was restricted at a prepenetration stage in entry and controlled by envelope sequences. Our results suggest that primary macrophages are sensitive to cell-to-cell but not to virion-to-cell fusion induced by the envelope glycoproteins of several T-cell tropic HIV-1 strains.
Authors:
G Simmons; A McKnight; Y Takeuchi; H Hoshino; P R Clapham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  209     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-11     Completed Date:  1995-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  696-700     Citation Subset:  IM; X    
Affiliation:
Virology Laboratory, Chester Beatty Laboratories, Institute of Cancer Research, London.
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MeSH Terms
Descriptor/Qualifier:
Cell Communication
Cell Fusion*
Cell Line
Cell-Free System
Gene Products, env / chemistry,  metabolism*
Giant Cells / cytology
HIV-1 / physiology*
Humans
Macrophages / cytology,  physiology*,  virology
T-Lymphocytes / physiology*,  virology
Virion / physiology
Virus Replication*
Chemical
Reg. No./Substance:
0/Gene Products, env

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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