| Cell to aperture interaction in patch-clamp chips visualized by fluorescence microscopy and focused-ion beam sections. | |
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MedLine Citation:
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PMID: 21391207 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Patch-clamp is an important method to monitor the electrophysiological activity of cells and the role of pharmacological compounds on specific ion channel proteins. In recent years planar patch-clamp chips have been developed as a higher throughput approach to the established glass-pipette method. However, proper conditions to optimize the high resistance cell-to-probe seals required to measure the small currents resulting from ion channel activity are still the subject of conjecture. Here, we report on the design of multiple-aperture (sieve) chips to rapidly facilitate assessment of cell-to-aperture interactions in statistically significant numbers. We propose a method to prescreen the quality of seals based on a dye loading protocol through apertures in the chip and subsequent evaluation with fluorescence confocal microscopy. We also show the first scanning electron micrograph of a focused ion beam section of a cell in a patch-clamp chip aperture. Biotechnol. Bioeng. © 2011 Wiley Periodicals, Inc. |
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Authors:
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Christophe Py; Danish Salim; Robert Monette; Tanya Comas; Jeffrey Fraser; Dolores Martinez; Marzia Martina; Geoffrey Mealing |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-9 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: - ISSN: 1097-0290 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Institute for Microstructural Sciences, National Research Council of Canada, 1200 Montreal Rd., Ottawa Ontario K1A0R6 Canada. christophe.py@nrc.ca. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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