| Cell-to-cell transmission of α-synuclein aggregates. | |
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MedLine Citation:
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PMID: 22528101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is now recognized that the cell-to-cell transmission of misfolded proteins such as α-synuclein contributes to the neurodegenerative phenotype in neurological disorders such as idiopathic Parkinson's disease, Dementia with Lewy bodies, and Parkinson's disease dementia. Thus, establishing cell-based models for the transmission of α-synuclein is of importance to understand the mechanisms of neurodegeneration in these disorders and to develop new therapies. Here we describe methods to study the neuron-to-neuron propagation of α-synuclein in an in vitro setting that also has in vivo applications. |
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Authors:
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Seung-Jae Lee; Paula Desplats; He-Jin Lee; Brian Spencer; Eliezer Masliah |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 849 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-04-24 Completed Date: 2012-08-08 Revised Date: 2013-05-02 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 347-59 Citation Subset: IM |
Affiliation:
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Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Communication* Cell Proliferation Centrifugation Enzyme-Linked Immunosorbent Assay Genetic Vectors / genetics HEK293 Cells Humans Lentivirus / genetics Mice Neural Stem Cells / cytology Protein Folding Protein Multimerization* Protein Structure, Secondary Protein Transport Staining and Labeling Transfection alpha-Synuclein / chemistry*, genetics, isolation & purification |
| Grant Support | |
ID/Acronym/Agency:
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AG 03097/AG/NIA NIH HHS; AG010435/AG/NIA NIH HHS; AG022074/AG/NIA NIH HHS; AG18440/AG/NIA NIH HHS; NS057096/NS/NINDS NIH HHS; R37 AG018440/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/alpha-Synuclein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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