Document Detail


Cell-to-cell transmission of α-synuclein aggregates.
MedLine Citation:
PMID:  22528101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is now recognized that the cell-to-cell transmission of misfolded proteins such as α-synuclein contributes to the neurodegenerative phenotype in neurological disorders such as idiopathic Parkinson's disease, Dementia with Lewy bodies, and Parkinson's disease dementia. Thus, establishing cell-based models for the transmission of α-synuclein is of importance to understand the mechanisms of neurodegeneration in these disorders and to develop new therapies. Here we describe methods to study the neuron-to-neuron propagation of α-synuclein in an in vitro setting that also has in vivo applications.
Authors:
Seung-Jae Lee; Paula Desplats; He-Jin Lee; Brian Spencer; Eliezer Masliah
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  849     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-08-08     Revised Date:  2013-05-02    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  347-59     Citation Subset:  IM    
Affiliation:
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication*
Cell Proliferation
Centrifugation
Enzyme-Linked Immunosorbent Assay
Genetic Vectors / genetics
HEK293 Cells
Humans
Lentivirus / genetics
Mice
Neural Stem Cells / cytology
Protein Folding
Protein Multimerization*
Protein Structure, Secondary
Protein Transport
Staining and Labeling
Transfection
alpha-Synuclein / chemistry*,  genetics,  isolation & purification
Grant Support
ID/Acronym/Agency:
AG 03097/AG/NIA NIH HHS; AG010435/AG/NIA NIH HHS; AG022074/AG/NIA NIH HHS; AG18440/AG/NIA NIH HHS; NS057096/NS/NINDS NIH HHS; R37 AG018440/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/alpha-Synuclein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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